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Sample GSM2096889 Query DataSets for GSM2096889
Status Public on Nov 23, 2017
Title RAW-264.7 FoxQ1, clone 7.4
Sample type RNA
 
Source name RAW-264.7 macrophage/monocyte cell line
Organism Mus musculus
Characteristics cell line: RAW-264.7
transfection: mouse FoxQ1 expression construct
Treatment protocol No additional treatments
Growth protocol RAW-264.7 stable transfectants were propagated in DMEM+Glutamax medium (ThermoFisher Scientific) supplemented with 10% fetal bovine serum, 1% pen/strep and 6 µg/ml blasticidin S
Extracted molecule total RNA
Extraction protocol Isolation of total RNA from cells was performed using E.Z.N.A. total RNA Kit I (Omega bio-tek) according to manufacturer’s recommendation
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
 
Hybridization protocol Hybridization (16h x 45°C) was processed according to the standard Affymetrix protocol.
Scan protocol Affymetrix GeneArray Scanner3000
Data processing The data were analyzed with a commercial software called JMP Genomics, version 5, from SAS. Gene expression profiling was performed using arrays of human mouse430_2-type from Affymetrix. A Custom CDF Version 13 with Entrez based gene definitions was used to annotate the arrays. The Raw fluorescence intensity values were normalized applying quantile normalization, RMA background correction and Medianpolish Probeset Summary
 
Submission date Mar 23, 2016
Last update date Nov 23, 2017
Contact name Carsten Sticht
Organization name University Heidelberg
Department ZMF
Street address Theodor-Kutzer-Ufer
City Mannheim
ZIP/Postal code 68169
Country Germany
 
Platform ID GPL14661
Series (1)
GSE79521 Expression data from RAW-264.7 cells transfected with empty vector and mouse FoxQ1 expression construct

Data table header descriptions
ID_REF
VALUE RMA signal intensity

Data table
ID_REF VALUE
100009600_at 7.122226912
100012_at 6.102391208
100017_at 8.985124025
100019_at 8.713070052
100034251_at 9.629484647
100036521_at 8.798469352
100037258_at 8.816999069
100037278_at 8.086102078
100038570_at 7.231698495
100038635_at 6.314735901
100038680_at 6.703531222
100038887_at 12.85732648
100038959_at 8.145482226
100039026_at 7.823710727
100039027_at 6.182527587
100039094_at 7.184246976
100039235_at 7.409791581
100039282_at 5.959027409
100039284_at 7.138935991
100039307_at 6.806679583

Total number of rows: 16556

Table truncated, full table size 343 Kbytes.




Supplementary file Size Download File type/resource
GSM2096889_Kzhyshkowska_081210_FoxQ1_Klon_7.4_Mouse430_2_.CEL.gz 3.6 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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