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Sample GSM2096890 Query DataSets for GSM2096890
Status Public on Nov 23, 2017
Title RAW-264.7 FoxQ1, clone 7.5
Sample type RNA
 
Source name RAW-264.7 macrophage/monocyte cell line
Organism Mus musculus
Characteristics cell line: RAW-264.7
transfection: mouse FoxQ1 expression construct
Treatment protocol No additional treatments
Growth protocol RAW-264.7 stable transfectants were propagated in DMEM+Glutamax medium (ThermoFisher Scientific) supplemented with 10% fetal bovine serum, 1% pen/strep and 6 µg/ml blasticidin S
Extracted molecule total RNA
Extraction protocol Isolation of total RNA from cells was performed using E.Z.N.A. total RNA Kit I (Omega bio-tek) according to manufacturer’s recommendation
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
 
Hybridization protocol Hybridization (16h x 45°C) was processed according to the standard Affymetrix protocol.
Scan protocol Affymetrix GeneArray Scanner3000
Data processing The data were analyzed with a commercial software called JMP Genomics, version 5, from SAS. Gene expression profiling was performed using arrays of human mouse430_2-type from Affymetrix. A Custom CDF Version 13 with Entrez based gene definitions was used to annotate the arrays. The Raw fluorescence intensity values were normalized applying quantile normalization, RMA background correction and Medianpolish Probeset Summary
 
Submission date Mar 23, 2016
Last update date Nov 23, 2017
Contact name Carsten Sticht
Organization name University Heidelberg
Department ZMF
Street address Theodor-Kutzer-Ufer
City Mannheim
ZIP/Postal code 68169
Country Germany
 
Platform ID GPL14661
Series (1)
GSE79521 Expression data from RAW-264.7 cells transfected with empty vector and mouse FoxQ1 expression construct

Data table header descriptions
ID_REF
VALUE RMA signal intensity

Data table
ID_REF VALUE
100009600_at 7.181182554
100012_at 6.094803316
100017_at 9.027202563
100019_at 8.607958193
100034251_at 9.014950905
100036521_at 8.774556125
100037258_at 9.021288787
100037278_at 8.235074267
100038570_at 7.204253832
100038635_at 6.443121472
100038680_at 6.617677284
100038887_at 12.90142098
100038959_at 8.282491518
100039026_at 8.019129152
100039027_at 6.118331074
100039094_at 7.171034298
100039235_at 7.403249446
100039282_at 5.888610991
100039284_at 7.127422183
100039307_at 6.830490342

Total number of rows: 16556

Table truncated, full table size 343 Kbytes.




Supplementary file Size Download File type/resource
GSM2096890_Kzhyshkowska_081210_FoxQ1_Klon_7.5_Mouse430_2_.CEL.gz 3.6 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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