|
| Status |
Public on Aug 04, 2007 |
| Title |
CAD_T3 |
| Sample type |
RNA |
| |
|
| Source name |
mouse neuroblastoma cell CAD
|
| Organism |
Mus musculus |
| Characteristics |
cultured mouse neuroblastoma cell CAD
cultured mouse neuroblastoma cell CAD
|
| Treatment protocol |
CAD cell line (Qi et al., 1997) was propagated in DMEM supplemented with 10% fetal bovine serum (FBS) or Fetaclone III and 1% penicillin/streptomycin, transfected RIPmiR-124a-2 plasmid, expressing miR-124a-2.
|
| Growth protocol |
CAD cell line (Qi et al., 1997) was propagated in DMEM supplemented with 10% fetal bovine serum (FBS) or Fetaclone III and 1% penicillin/streptomycin.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0 Array.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000.
|
| Description |
CAD transfected with RIPmiR-124a-2 plasmid, expressing miR-124a-2, biological rep3
|
| Data processing |
The data were were normalized by robust multi-chip analysis (RMA) (Bioconductor release 1.9) using PM-only models.
|
| |
|
| Submission date |
Jul 16, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Jiangwen Zhang |
| E-mail(s) |
jzhang@cgr.harvard.edu
|
| Organization name |
Harvard University
|
| Department |
FAS Center for Systems Biology
|
| Street address |
7 Divinity Ave
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02138 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE8498 |
The MicroRNA miR-124 Promotes Neuronal Differentiation by Triggering Brain-Specific Alternative Pre-mRNA Splicing |
|
| Relations |
| Reanalyzed by |
GSE119085 |
