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Sample GSM211300 Query DataSets for GSM211300
Status Public on Jul 19, 2007
Title Macrophage smi105
Sample type RNA
 
Source name 16 week old apoE-deficient F2 female mouse from an AKRxDBA/2 intercross
Organism Mus musculus
Characteristics Female
Growth protocol Cells were cultured for 2 weeks in DMEM containing 10% FBS and 20% L-cell conditioned medium
Extracted molecule total RNA
Extraction protocol RNA extracted with RNAeasy minikit (Qiagen)
Label Biotin
Label protocol Biotin labeled cRNA was prepared from 1 ug RNA using the BioARray High Yield RNA Transcript labeling kit (Enzo)
 
Hybridization protocol Following fragmentation, 12 ug of cRNA was hybridized to Affymetrix 430v2 arrays according to Affymetric protocols
Scan protocol Arrays were scanned using the Hewlet-Packard GeneArray Scanner G2500A
Description Gene expression data from mouse bone marrow macrophages
Data processing The data were analyzed with Affymetrix Microarray Suite version 5.0 (MAS 5.0) using Refiner software (Genedata) using default settings. The normalization target was set to 500.
 
Submission date Jul 18, 2007
Last update date Aug 02, 2019
Contact name Jonathan David Smith
E-mail(s) smithj4@ccf.org
URL http://www.lerner.ccf.org/cellbio/smith/
Organization name Cleveland Clinic
Department Cell Biology
Lab Smith
Street address 9500 Euclid Avenue
City Cleveland
State/province OH
ZIP/Postal code 44195
Country USA
 
Platform ID GPL1261
Series (1)
GSE8512 Expression data from mouse bone marrow macrophages from a strain intercross
Relations
Reanalyzed by GSE119085
Reanalyzed by GSE135324

Data table header descriptions
ID_REF
VALUE MAS5.0 signal intensity
ABS_CALL

Data table
ID_REF VALUE ABS_CALL
AFFX-BioB-5_at 456.2 P
AFFX-BioB-M_at 806.1 P
AFFX-BioB-3_at 603.1 P
AFFX-BioC-5_at 1872 P
AFFX-BioC-3_at 2200.9 P
AFFX-BioDn-5_at 3580.1 P
AFFX-BioDn-3_at 6771.5 P
AFFX-CreX-5_at 21251.9 P
AFFX-CreX-3_at 24402.3 P
AFFX-DapX-5_at 4085.7 P
AFFX-DapX-M_at 8913.9 P
AFFX-DapX-3_at 14269.8 P
AFFX-LysX-5_at 793.1 P
AFFX-LysX-M_at 1002.3 P
AFFX-LysX-3_at 2619.7 P
AFFX-PheX-5_at 1279.2 P
AFFX-PheX-M_at 1711.1 P
AFFX-PheX-3_at 2117.7 P
AFFX-ThrX-5_at 1143.5 P
AFFX-ThrX-M_at 2299.5 P

Total number of rows: 45101

Table truncated, full table size 821 Kbytes.




Supplementary file Size Download File type/resource
GSM211300.CEL.gz 3.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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