|
| Status |
Public on Jul 19, 2007 |
| Title |
Macrophage 14smi009 |
| Sample type |
RNA |
| |
|
| Source name |
16 week old apoE-deficient F2 male mouse from an AKRxDBA/2 intercross
|
| Organism |
Mus musculus |
| Characteristics |
Male
|
| Growth protocol |
Cells were cultured for 2 weeks in DMEM containing 10% FBS and 20% L-cell conditioned medium
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extracted with RNAeasy minikit (Qiagen)
|
| Label |
Biotin
|
| Label protocol |
Biotin labeled cRNA was prepared from 1 ug RNA using the BioARray High Yield RNA Transcript labeling kit (Enzo)
|
| |
|
| Hybridization protocol |
Following fragmentation, 12 ug of cRNA was hybridized to Affymetrix 430v2 arrays according to Affymetric protocols
|
| Scan protocol |
Arrays were scanned using the Hewlet-Packard GeneArray Scanner G2500A
|
| Description |
Gene expression data from mouse bone marrow macrophages
|
| Data processing |
The data were analyzed with Affymetrix Microarray Suite version 5.0 (MAS 5.0) using Refiner software (Genedata) using default settings. The normalization target was set to 500.
|
| |
|
| Submission date |
Jul 18, 2007 |
| Last update date |
Aug 02, 2019 |
| Contact name |
Jonathan David Smith |
| E-mail(s) |
smithj4@ccf.org
|
| URL |
http://www.lerner.ccf.org/cellbio/smith/
|
| Organization name |
Cleveland Clinic
|
| Department |
Cell Biology
|
| Lab |
Smith
|
| Street address |
9500 Euclid Avenue
|
| City |
Cleveland |
| State/province |
OH |
| ZIP/Postal code |
44195 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE8512 |
Expression data from mouse bone marrow macrophages from a strain intercross |
|
| Relations |
| Reanalyzed by |
GSE119085 |
| Reanalyzed by |
GSE135324 |
