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Sample GSM211427 Query DataSets for GSM211427
Status Public on Jul 19, 2007
Title Macrophage 14smi114
Sample type RNA
 
Source name 16 week old apoE-deficient F2 male mouse from an AKRxDBA/2 intercross
Organism Mus musculus
Characteristics Male
Growth protocol Cells were cultured for 2 weeks in DMEM containing 10% FBS and 20% L-cell conditioned medium
Extracted molecule total RNA
Extraction protocol RNA extracted with RNAeasy minikit (Qiagen)
Label Biotin
Label protocol Biotin labeled cRNA was prepared from 1 ug RNA using the BioARray High Yield RNA Transcript labeling kit (Enzo)
 
Hybridization protocol Following fragmentation, 12 ug of cRNA was hybridized to Affymetrix 430v2 arrays according to Affymetric protocols
Scan protocol Arrays were scanned using the Hewlet-Packard GeneArray Scanner G2500A
Description Gene expression data from mouse bone marrow macrophages
Data processing The data were analyzed with Affymetrix Microarray Suite version 5.0 (MAS 5.0) using Refiner software (Genedata) using default settings. The normalization target was set to 500.
 
Submission date Jul 18, 2007
Last update date Aug 02, 2019
Contact name Jonathan David Smith
E-mail(s) smithj4@ccf.org
URL http://www.lerner.ccf.org/cellbio/smith/
Organization name Cleveland Clinic
Department Cell Biology
Lab Smith
Street address 9500 Euclid Avenue
City Cleveland
State/province OH
ZIP/Postal code 44195
Country USA
 
Platform ID GPL1261
Series (1)
GSE8512 Expression data from mouse bone marrow macrophages from a strain intercross
Relations
Reanalyzed by GSE119085
Reanalyzed by GSE135324

Data table header descriptions
ID_REF
VALUE MAS5.0 signal intensity
ABS_CALL

Data table
ID_REF VALUE ABS_CALL
AFFX-BioB-5_at 516.6 P
AFFX-BioB-M_at 865.8 P
AFFX-BioB-3_at 571.4 P
AFFX-BioC-5_at 1725.5 P
AFFX-BioC-3_at 1861.1 P
AFFX-BioDn-5_at 3095.3 P
AFFX-BioDn-3_at 6494.4 P
AFFX-CreX-5_at 20837.1 P
AFFX-CreX-3_at 24818.9 P
AFFX-DapX-5_at 2712.9 P
AFFX-DapX-M_at 5862.5 P
AFFX-DapX-3_at 8863.5 P
AFFX-LysX-5_at 447.1 P
AFFX-LysX-M_at 599.2 P
AFFX-LysX-3_at 1453.9 P
AFFX-PheX-5_at 666.4 P
AFFX-PheX-M_at 1080.2 P
AFFX-PheX-3_at 1063.1 P
AFFX-ThrX-5_at 899.1 P
AFFX-ThrX-M_at 1552.3 P

Total number of rows: 45101

Table truncated, full table size 822 Kbytes.




Supplementary file Size Download File type/resource
GSM211427.CEL.gz 3.5 Mb (ftp)(http) CEL
Processed data included within Sample table

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