|
| Status |
Public on Dec 26, 2007 |
| Title |
tumor tissue 6 weeks post virus injection mouse4 |
| Sample type |
RNA |
| |
|
| Source name |
human breast cancer implant in nude mice
|
| Organism |
Mus musculus |
| Characteristics |
tumor implant 6 weeks post virus injection
|
| Treatment protocol |
On day 30 after cell implantation, a single dose of virus (5 × 106 pfu of GLV-1h68 in 100 ml of PBS) was delivered via the femoral vein. Four mice per group were killed 3 and 6 weeks following GLV-1h68 injection. An identical number of control mice treated with PBS alone were killed in parallel.
|
| Growth protocol |
To evaluate the involvement of host mechanisms in the eradication of transplanted xenografts, we implanted human GI-101A cells (5 × 106 in 100 ml of PBS) s.c. on the right hind leg of 6-8-week-old female nude mice.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from excised tumors using a QIAGEN RNeasy Protect Mini Kit
|
| Label |
biotin
|
| Label protocol |
GeneChip® One-Cycle Target Labeling and Control Reagents (Affymetrix) according to manufacturer instructions
|
| |
|
| Hybridization protocol |
Hybridization as specified by Affymetrix using the DMSO protocol
|
| Scan protocol |
Fluidics station 450 used to stain and wash arrays. Chips were scan using GCOS 1.4
|
| Description |
tumor tissue 6 weeks post virus injection
|
| Data processing |
Array data analysis utilized the BRB array tool (http://linus.nci.nih.gov/BRB-ArrayTools.html) developed by Dr. Richard Simon and Amy Peng Lam at NIH. Data were filtered at intensity >10, excluding absent calls and normalized via median centering. Probes with missing value or notations with absent calls in >50% of the entire experiments were also excluded.
|
| |
|
| Submission date |
Jul 18, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Francesco Maria Marincola |
| E-mail(s) |
fmarincola@sidra.org
|
| Phone |
301-793-8210
|
| Organization name |
Sidra Medical and Research Center
|
| Street address |
Al Nasr Tower, AL Corniche Street, PO Box 26999
|
| City |
Doha |
| ZIP/Postal code |
PO Box 26999 |
| Country |
Qatar |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE8513 |
Eradication of Solid Human Tumors in Nude Mice with an Intravenously Injected Light-Emitting Oncolytic Vaccinia Virus |
|
| Relations |
| Reanalyzed by |
GSE119085 |
