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Sample GSM211453 Query DataSets for GSM211453
Status Public on Jul 18, 2007
Title Aldosterone-producing adenoma (APA12)
Sample type RNA
Source name Aldosterone-producing adenoma
Organism Homo sapiens
Characteristics Genotype: Homo sapiens
Extracted molecule total RNA
Extraction protocol The tissue was pulverized in liquid nitrogen to a powder. Total RNA was extracted using TRIzol Reagent (Invitrogen, Carlsbad, CA). The purity and integrity of the RNA were assessed by the Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA) and its quantity was determined by NanoDrop spectrophotometer (NanoDrop Technologies, Wilmington, DE).
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001,
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Affymetrix Human Genome U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description Gene expression data from aldosterone-producing adenoma
Data processing The data were analyzed with GeneSpring 7.3 using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1.
Submission date Jul 18, 2007
Last update date Aug 28, 2018
Contact name Ping Ye
Phone 706 721 8779
Fax 706 721 8360
Organization name Medical College of Georgia
Department Physiology
Lab CA 3093
Street address 1120 15th Street
City Augusta
State/province GA
ZIP/Postal code 30912
Country USA
Platform ID GPL570
Series (1)
GSE8514 Expression data from normal adrenal gland and aldosterone-producing adenoma
Reanalyzed by GSE64985
Reanalyzed by GSE119087

Data table header descriptions
VALUE Signal
ABS_CALL indicating whether the transcript was present (P), absent (A), or marginal (M)

Data table
AFFX-BioB-5_at 249.605 P 0.000753643
AFFX-BioB-M_at 310.365 P 0.000195116
AFFX-BioB-3_at 187.946 P 0.000445901
AFFX-BioC-5_at 505.69 P 0.000224668
AFFX-BioC-3_at 662.889 P 5.16732e-05
AFFX-BioDn-5_at 1323.49 P 5.16732e-05
AFFX-BioDn-3_at 4384.84 P 0.000389797
AFFX-CreX-5_at 10466.5 P 5.16732e-05
AFFX-CreX-3_at 11315.2 P 4.42873e-05
AFFX-DapX-5_at 9.00691 A 0.275146
AFFX-DapX-M_at 3.25726 A 0.860518
AFFX-DapX-3_at 3.11354 A 0.860518
AFFX-LysX-5_at 11.1442 A 0.239044
AFFX-LysX-M_at 4.01027 A 0.724854
AFFX-LysX-3_at 3.22084 A 0.645547
AFFX-PheX-5_at 2.67102 A 0.794268
AFFX-PheX-M_at 2.36402 A 0.794268
AFFX-PheX-3_at 5.80758 A 0.772364
AFFX-ThrX-5_at 8.45352 A 0.749204
AFFX-ThrX-M_at 2.02988 A 0.724854

Total number of rows: 54675

Table truncated, full table size 1620 Kbytes.

Supplementary file Size Download File type/resource
GSM211453.CEL.gz 5.2 Mb (ftp)(http) CEL
GSM211453.CHP.gz 295.6 Kb (ftp)(http) CHP
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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