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Sample GSM211457 Query DataSets for GSM211457
Status Public on Jul 18, 2007
Title Aldosterone-producing adenoma (APA4)
Sample type RNA
 
Source name Aldosterone-producing adenoma
Organism Homo sapiens
Characteristics Genotype: Homo sapiens
Extracted molecule total RNA
Extraction protocol The tissue was pulverized in liquid nitrogen to a powder. Total RNA was extracted using TRIzol Reagent (Invitrogen, Carlsbad, CA). The purity and integrity of the RNA were assessed by the Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA) and its quantity was determined by NanoDrop spectrophotometer (NanoDrop Technologies, Wilmington, DE).
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001,
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Affymetrix Human Genome U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description Gene expression data from aldosterone-producing adenoma
Data processing The data were analyzed with GeneSpring 7.3 using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1.
 
Submission date Jul 18, 2007
Last update date Aug 28, 2018
Contact name Ping Ye
E-mail(s) pye@mcg.edu
Phone 706 721 8779
Fax 706 721 8360
Organization name Medical College of Georgia
Department Physiology
Lab CA 3093
Street address 1120 15th Street
City Augusta
State/province GA
ZIP/Postal code 30912
Country USA
 
Platform ID GPL570
Series (1)
GSE8514 Expression data from normal adrenal gland and aldosterone-producing adenoma
Relations
Reanalyzed by GSE64985
Reanalyzed by GSE119087

Data table header descriptions
ID_REF
VALUE Signal
ABS_CALL indicating whether the transcript was present (P), absent (A), or marginal (M)
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 2024.14 P 7.00668e-05
AFFX-BioB-M_at 2910.29 P 4.42873e-05
AFFX-BioB-3_at 1284.06 P 5.16732e-05
AFFX-BioC-5_at 5151.73 P 4.42873e-05
AFFX-BioC-3_at 5394.01 P 4.42873e-05
AFFX-BioDn-5_at 8573.26 P 4.42873e-05
AFFX-BioDn-3_at 31045.7 P 4.42873e-05
AFFX-CreX-5_at 60922.1 P 5.16732e-05
AFFX-CreX-3_at 65394.8 P 4.42873e-05
AFFX-DapX-5_at 46.117 A 0.205712
AFFX-DapX-M_at 10.4311 A 0.814869
AFFX-DapX-3_at 4.69156 A 0.981728
AFFX-LysX-5_at 41.6264 A 0.5
AFFX-LysX-M_at 34.6643 A 0.411373
AFFX-LysX-3_at 14.2464 A 0.455413
AFFX-PheX-5_at 4.35794 A 0.960352
AFFX-PheX-M_at 6.10171 A 0.916408
AFFX-PheX-3_at 9.50375 A 0.794268
AFFX-ThrX-5_at 3.41474 A 0.989683
AFFX-ThrX-M_at 11.4668 A 0.699394

Total number of rows: 54675

Table truncated, full table size 1605 Kbytes.




Supplementary file Size Download File type/resource
GSM211457.CEL.gz 4.1 Mb (ftp)(http) CEL
GSM211457.CHP.gz 302.3 Kb (ftp)(http) CHP
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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