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Sample GSM212558 Query DataSets for GSM212558
Status Public on Jul 25, 2007
Title Gene expression profiling of Phgdh knockout embryo wild1
Sample type RNA
 
Source name Head RNA of littermate wild@E13.5 No.1
Organism Mus musculus
Characteristics ES cells: E14 (129P2/OlaHsd background),
Genotype: Wild (Phgdh+/+), C57BL/6J background,
Gestation: 13.5 days,
Tissue: head
Biomaterial provider Shigeki Furuya
Treatment protocol Targeted disruption of D-3-Phosphoglycerate dehydrogenase (Phgdh; EC 1.1.1.95) in mice.
Growth protocol None
Extracted molecule total RNA
Extraction protocol Total RNA from embryo heads was extracted and purified using RNeasy Lipid Tissue kit (Qiagen Inc) according to the manufacturer’s instructions.
Label Biotin
Label protocol Labeling was carried out using Affymetrix GeneChip® One-Cycle Target Labeling kit, according to manufactuer's protocol.
 
Hybridization protocol Hybridization was carried out for 16 hr at 45 ºC according the Affymetrix GeneChip® Manual.
Scan protocol Scanning was carried out using Affymetrix® GeneChip® Scanner with GCOS software according to manufactuer's protocol.
Description Affymetrix GeneChip® Mouse Genome 430 2.0 Array
Data processing Fluorescent data were processed using the Microarray Suite (MAS) 5.0 software (Affymetrix) and then were transferred to GeneSpring 7.0 software (Agilent).
 
Submission date Jul 23, 2007
Last update date Aug 14, 2011
Contact name Shigeki Furuya
E-mail(s) shigekifur@brs.kyushu-u.ac.jp
Organization name Kyushu University
Department Bio-Architecture Center
Lab Furuya Laboratory
Street address 6-10-1 Hakozaki, Higashiku
City Fukuoka
State/province Fukuoka
ZIP/Postal code 812-8581
Country Japan
 
Platform ID GPL1261
Series (1)
GSE8555 Genome-wide analysis of Phgdh inactivation in murine embryonic head

Data table header descriptions
ID_REF
VALUE Signal intensity
ABS_CALL Absolute Call: A (Absent), M (Marginal), or P (Present)
DETECTION P-VALUE P-value generated by MAS5

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 96.5771 P 0.000753643
AFFX-BioB-M_at 131.152 P 0.000753643
AFFX-BioB-3_at 83.3212 P 0.000195116
AFFX-BioC-5_at 236.156 P 0.000109401
AFFX-BioC-3_at 195.253 P 6.02111e-05
AFFX-BioDn-5_at 276.568 P 4.42873e-05
AFFX-BioDn-3_at 1024.62 P 6.02111e-05
AFFX-CreX-5_at 3163.78 P 5.16732e-05
AFFX-CreX-3_at 3765.09 P 4.42873e-05
AFFX-DapX-5_at 5.70017 A 0.250796
AFFX-DapX-M_at 0.795092 A 0.860518
AFFX-DapX-3_at 1.49202 A 0.945787
AFFX-LysX-5_at 0.881118 A 0.470241
AFFX-LysX-M_at 1.91767 A 0.699409
AFFX-LysX-3_at 1.05174 A 0.617401
AFFX-PheX-5_at 0.34596 A 0.937071
AFFX-PheX-M_at 0.615857 A 0.904333
AFFX-PheX-3_at 1.49449 A 0.897835
AFFX-ThrX-5_at 1.00832 A 0.860518
AFFX-ThrX-M_at 1.68061 A 0.749204

Total number of rows: 45101

Table truncated, full table size 1374 Kbytes.




Supplementary file Size Download File type/resource
GSM212558.CHP.gz 10.6 Mb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file
Raw data not provided for this record

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