|
| Status |
Public on Jul 25, 2007 |
| Title |
Gene expression profiling of Phgdh knockout embryo KO2 |
| Sample type |
RNA |
| |
|
| Source name |
Head RNA of Phgdh Knockout@E13.5 No.2
|
| Organism |
Mus musculus |
| Characteristics |
ES cells: E14 (129P2/OlaHsd background), Genotype: Knockout (Phgdh-/-), C57BL/6J background, Gestation: 13.5 days, Tissue: head
|
| Biomaterial provider |
Shigeki Furuya
|
| Treatment protocol |
Targeted disruption of D-3-Phosphoglycerate dehydrogenase (Phgdh; EC 1.1.1.95) in mice.
|
| Growth protocol |
None
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from embryo heads was extracted and purified using RNeasy Lipid Tissue kit (Qiagen Inc) according to the manufacturer’s instructions.
|
| Label |
Biotin
|
| Label protocol |
Labeling was carried out using Affymetrix GeneChip® One-Cycle Target Labeling kit, according to manufactuer's protocol.
|
| |
|
| Hybridization protocol |
Hybridization was carried out for 16 hr at 45 ºC according the Affymetrix GeneChip® Manual.
|
| Scan protocol |
Scanning was carried out using Affymetrix® GeneChip® Scanner with GCOS software according to manufactuer's protocol.
|
| Description |
Affymetrix GeneChip® Mouse Genome 430 2.0 Array
|
| Data processing |
Fluorescent data were processed using the Microarray Suite (MAS) 5.0 software (Affymetrix) and then were transferred to GeneSpring 7.0 software (Agilent).
|
| |
|
| Submission date |
Jul 23, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Shigeki Furuya |
| E-mail(s) |
shigekifur@brs.kyushu-u.ac.jp
|
| Organization name |
Kyushu University
|
| Department |
Bio-Architecture Center
|
| Lab |
Furuya Laboratory
|
| Street address |
6-10-1 Hakozaki, Higashiku
|
| City |
Fukuoka |
| State/province |
Fukuoka |
| ZIP/Postal code |
812-8581 |
| Country |
Japan |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE8555 |
Genome-wide analysis of Phgdh inactivation in murine embryonic head |
|
| Relations |
| Reanalyzed by |
GSE119085 |
