|
Status |
Public on Jul 25, 2007 |
Title |
Gene expression profiling of Phgdh knockout embryo KO3 |
Sample type |
RNA |
|
|
Source name |
Head RNA of Phgdh Knockout@E13.5 No.3
|
Organism |
Mus musculus |
Characteristics |
ES cells: E14 (129P2/OlaHsd background), Genotype: Knockout (Phgdh-/-), C57BL/6J background, Gestation: 13.5 days, Tissue: head
|
Biomaterial provider |
Shigeki Furuya
|
Treatment protocol |
Targeted disruption of D-3-Phosphoglycerate dehydrogenase (Phgdh; EC 1.1.1.95) in mice.
|
Growth protocol |
None
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA from embryo heads was extracted and purified using RNeasy Lipid Tissue kit (Qiagen Inc) according to the manufacturer’s instructions.
|
Label |
Biotin
|
Label protocol |
Labeling was carried out using Affymetrix GeneChip® One-Cycle Target Labeling kit, according to manufactuer's protocol.
|
|
|
Hybridization protocol |
Hybridization was carried out for 16 hr at 45 ºC according the Affymetrix GeneChip® Manual.
|
Scan protocol |
Scanning was carried out using Affymetrix® GeneChip® Scanner with GCOS software according to manufactuer's protocol.
|
Description |
Affymetrix GeneChip® Mouse Genome 430 2.0 Array
|
Data processing |
Fluorescent data were processed using the Microarray Suite (MAS) 5.0 software (Affymetrix) and then were transferred to GeneSpring 7.0 software (Agilent).
|
|
|
Submission date |
Jul 23, 2007 |
Last update date |
Aug 28, 2018 |
Contact name |
Shigeki Furuya |
E-mail(s) |
shigekifur@brs.kyushu-u.ac.jp
|
Organization name |
Kyushu University
|
Department |
Bio-Architecture Center
|
Lab |
Furuya Laboratory
|
Street address |
6-10-1 Hakozaki, Higashiku
|
City |
Fukuoka |
State/province |
Fukuoka |
ZIP/Postal code |
812-8581 |
Country |
Japan |
|
|
Platform ID |
GPL1261 |
Series (1) |
GSE8555 |
Genome-wide analysis of Phgdh inactivation in murine embryonic head |
|
Relations |
Reanalyzed by |
GSE119085 |