|
Status |
Public on Apr 26, 2016 |
Title |
Chow CTRL biological rep. 1 |
Sample type |
RNA |
|
|
Source name |
kidney, chow diet, CTRL mice
|
Organism |
Mus musculus |
Characteristics |
tissue: Kidney genotype: control
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Sense target biotin labeling according to the Mouse 2.0 ST Whole Gene Array from Affymetrix
|
|
|
Hybridization protocol |
Following fragmentation, 4.25 ug of DNA were hybridized for 17 hr at 45C. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
Scan protocol |
GeneChips were scanned using the Affymetrix Scanner 3000-7G.
|
Data processing |
The data were analyzed at the Life Sciences Training Facility of the University of Basel, using the PARTEK Software Suite and Bioconductor.
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|
|
Submission date |
Apr 25, 2016 |
Last update date |
Apr 26, 2016 |
Contact name |
Christoph Handschin |
Organization name |
Biozentrum, University of Basel
|
Street address |
Spitalstrasse 41
|
City |
Basel |
ZIP/Postal code |
4056 |
Country |
Switzerland |
|
|
Platform ID |
GPL16570 |
Series (1) |
GSE80618 |
Loss of renal tubular PGC-1α exacerbates diet-induced renal steatosis and age-related urinary sodium excretion in mice |
|