|
| Status |
Public on Apr 26, 2016 |
| Title |
HFD NiPKO biological rep. 4 |
| Sample type |
RNA |
| |
|
| Source name |
kidney, HFD, NiPKO mice
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Kidney
genotype: PGC-1alpha knockout
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Sense target biotin labeling according to the Mouse 2.0 ST Whole Gene Array from Affymetrix
|
| |
|
| Hybridization protocol |
Following fragmentation, 4.25 ug of DNA were hybridized for 17 hr at 45C. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix Scanner 3000-7G.
|
| Data processing |
The data were analyzed at the Life Sciences Training Facility of the University of Basel, using the PARTEK Software Suite and Bioconductor.
|
| |
|
| Submission date |
Apr 25, 2016 |
| Last update date |
Apr 26, 2016 |
| Contact name |
Christoph Handschin |
| Organization name |
Biozentrum, University of Basel
|
| Street address |
Spitalstrasse 41
|
| City |
Basel |
| ZIP/Postal code |
4056 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL16570 |
| Series (1) |
| GSE80618 |
Loss of renal tubular PGC-1α exacerbates diet-induced renal steatosis and age-related urinary sodium excretion in mice |
|
