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Sample GSM213376 Query DataSets for GSM213376
Status Public on Jul 31, 2007
Title Cervical carcinoma 2 Post-treatment
Sample type RNA
 
Source name Cervical carcinoma Punch biopsy after hydralazine and magnesium valproate treatment
Organism Homo sapiens
Characteristics Punch biopsy were taken from primary cervical tumors at diagnosis and at day 8 of treatment with hydralazine and magnesium valproate
Treatment protocol Patients received a single oral dose of 500 mg of sulphamethazine early in the morning, and urine was collected within the ensuing 6 h for phenotyping of acetylator status as reported. Afterward, patients began treatment (day –7) with a daily dose of a slow-release formulation of hydralazine tablets containing either 182 mg for rapid, or 83 mg for slow acetylators. Magnesium valproate tablets of 700 mg were also administered as a slow-release formulation at a dose of 30 mg/kg t.i.d. Both were administered until intracavitary therapy was completed. Punch biopsies were taken from primary cervical tumors at diagnosis and at day 8 of treatment with hydralazine and magnesium valproate prior to the first dose of cisplatin and external radiation. Part of the biopsy was sent to the Institution’s Pathology Department for routine hematoxilin and eosin evaluation. The remaining biopsy specimen was immediately frozen at –70°C for biological analyses.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions
Label Biotin
Label protocol Biotinylated cRNA was prepared according to the standard CodeLink protocol from 2 ug total RNA
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 37C on Human Whole Genome CodeLink Bioarray. Stain and post-hybridization washes were performed according to the manufacturer's instructions.
Scan protocol Bioarrays were scanned with a GenePix Personal 4100A scanner.
Description None
Data processing Expression values were globally normalized to the median expression value of the whole array spots. To circumvent division by zero, values below three times background value were taken as 5.
 
Submission date Jul 26, 2007
Last update date Aug 14, 2011
Contact name Carlos Pérez-Plasencia
E-mail(s) car_plas@yahoo.com
Phone +(52) 55 56280400
Fax +(52) 55 56280486
URL http://www.incan.edu.mx/
Organization name INSTITUTO NACIONAL DE CANCEROLOGIA
Department BASIC RESEARCH
Lab EPIGENETICS
Street address Av. San Fernando 22
City México
State/province D.F.
ZIP/Postal code 14080
Country Mexico
 
Platform ID GPL2895
Series (1)
GSE8604 Epigenetic therapy with hydralazine and magnesium valproate added to chemoradiation in cervical carcinoma.

Data table header descriptions
ID_REF
VALUE Normalized Signal intensity given by CodeLink Expression Analysis Software v4.1

Data table
ID_REF VALUE
45005 33.96
83005 40.71
129005 49.22
167005 51.50
213005 41.20
251005 40.41
297005 35.13
335005 49.20
381005 47.34
419005 40.60
465005 43.68
503005 22.31
45089 76.61
83089 40.42
129089 71.64
167089 69.71
213089 60.77
251089 76.36
297089 75.33
335089 63.88

Total number of rows: 54359

Table truncated, full table size 636 Kbytes.




Supplementary file Size Download File type/resource
GSM213376.TXT.gz 4.0 Mb (ftp)(http) TXT
Processed data included within Sample table

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