|
| Status |
Public on Jul 31, 2007 |
| Title |
Cervical carcinoma 16 Pre-treatment |
| Sample type |
RNA |
| |
|
| Source name |
Cervical carcinoma Punch biopsy before hydralazine and magnesium valproate treatment
|
| Organism |
Homo sapiens |
| Characteristics |
Gender: Female
Tumor Stage: Cervical carcinoma IIIB (FIGO)
|
| Treatment protocol |
Patients received a single oral dose of 500 mg of sulphamethazine early in the morning, and urine was collected within the ensuing 6 h for phenotyping of acetylator status as reported. Afterward, patients began treatment (day –7) with a daily dose of a slow-release formulation of hydralazine tablets containing either 182 mg for rapid, or 83 mg for slow acetylators. Magnesium valproate tablets of 700 mg were also administered as a slow-release formulation at a dose of 30 mg/kg t.i.d. Both were administered until intracavitary therapy was completed. Punch biopsies were taken from primary cervical tumors at diagnosis and at day 8 of treatment with hydralazine and magnesium valproate prior to the first dose of cisplatin and external radiation. Part of the biopsy was sent to the Institution’s Pathology Department for routine hematoxilin and eosin evaluation. The remaining biopsy specimen was immediately frozen at –70°C for biological analyses.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA was prepared according to the standard CodeLink protocol from 2 ug total RNA
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 37C on Human Whole Genome CodeLink Bioarray. Stain and post-hybridization washes were performed according to the manufacturer's instructions.
|
| Scan protocol |
Bioarrays were scanned with a GenePix Personal 4100A scanner.
|
| Description |
None
|
| Data processing |
Expression values were globally normalized to the median expression value of the whole array spots. To circumvent division by zero, values below three times background value were taken as 5.
|
| |
|
| Submission date |
Jul 26, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Carlos Pérez-Plasencia |
| E-mail(s) |
car_plas@yahoo.com
|
| Phone |
+(52) 55 56280400
|
| Fax |
+(52) 55 56280486
|
| URL |
http://www.incan.edu.mx/
|
| Organization name |
INSTITUTO NACIONAL DE CANCEROLOGIA
|
| Department |
BASIC RESEARCH
|
| Lab |
EPIGENETICS
|
| Street address |
Av. San Fernando 22
|
| City |
México |
| State/province |
D.F. |
| ZIP/Postal code |
14080 |
| Country |
Mexico |
| |
|
| Platform ID |
GPL2895 |
| Series (1) |
| GSE8604 |
Epigenetic therapy with hydralazine and magnesium valproate added to chemoradiation in cervical carcinoma. |
|
