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Sample GSM213617 Query DataSets for GSM213617
Status Public on May 29, 2008
Title SG_w1118_14APF_rep1
Sample type RNA
 
Source name salivary gland, heat shocked, dissected 14 hours after puparium formation
Organism Drosophila melanogaster
Characteristics Salivary glands of strain w1118, dissected at 14 hours after puparium formation.
Treatment protocol Animals were subjected to a 30-min heat shock treatment at 37 °C at 9.5 hours after puparium formation. Salivary glands were dissected for RNA extraction at 14 hours after puparium formation.
Growth protocol Animals were kept at 25 °C, except during heat shock period.
Extracted molecule total RNA
Extraction protocol Salivary glands were dissected on ice and directly transferred to TRIzol Reagent (Invitrogen, Carlsbad, California). To support lysis of the tissue, the mixture was subjected to vigorous vortexing. Total RNA was isolated according to the instructions of the manufacturer. As a last step of the extraction procedure, the RNA was purified using an RNeasy Minikit (Quiagen, Hilden, Germany).
Label biotin
Label protocol Biotin-labeled cRNA was produced using the One-Cycle Eukaryotic Target Labeling Assay recommended by Affymetrix. Labeling was carried out exactly as specified in the instructions manual, section 2 (http://www.affymetrix.com/support/technical/manual/expression_manual.affx).
 
Hybridization protocol Hybridization was performed following the instructions provided in the Affymetrix Manual (Section 2), using a Fluidics Station 450 and the Midi_euk2 v3 protocol.
Scan protocol GeneChips were scanned using the Affymetrix Genechip 3000 Scanner 7G and Genechip Operation Software (GCOS) v. 1.4.
Description Salivary glands from w1118 animals heat shocked at 9.5 hours after puparium formation and dissected at 14 hours after puparium formation.
Data processing The data were processed and normalized using dChip 2006. The median intensity of the baseline assay used for normalization was 150.
 
Submission date Jul 27, 2007
Last update date Aug 14, 2011
Contact name Michael Lehmann
E-mail(s) mlehmann@uark.edu
Phone 479-575-3688
Organization name University of Arkansas
Department Biological Sciences
Street address SCEN 601
City Fayetteville
State/province AR
ZIP/Postal code 72701
Country USA
 
Platform ID GPL1322
Series (2)
GSE8619 Senseless-responsive genes in larval salivary glands of late Drosophila prepupae
GSE8623 Senseless-responsive and GAL4-responsive genes in larval salivary glands of late Drosophila prepupae

Data table header descriptions
ID_REF
VALUE dChip-calculated signal intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 902.281
AFFX-BioB-M_at 1086.11
AFFX-BioB-3_at 1021.73
AFFX-BioC-5_at 2105.06
AFFX-BioC-3_at 2515.25
AFFX-BioDn-5_at 3286.71
AFFX-BioDn-3_at 5830.7
AFFX-CreX-5_at 11200.3
AFFX-CreX-3_at 12769.8
AFFX-DapX-5_at 1710.67
AFFX-DapX-M_at 2961.71
AFFX-DapX-3_at 3843.24
AFFX-LysX-5_at 250.83
AFFX-LysX-M_at 303.331
AFFX-LysX-3_at 868.128
AFFX-PheX-5_at 519.501
AFFX-PheX-M_at 607.37
AFFX-PheX-3_at 748.076
AFFX-ThrX-5_at 484.1
AFFX-ThrX-M_at 782.471

Total number of rows: 18952

Table truncated, full table size 358 Kbytes.




Supplementary file Size Download File type/resource
GSM213617.CEL.gz 3.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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