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Sample GSM213694 Query DataSets for GSM213694
Status Public on Aug 10, 2007
Title no_pre_no_restimulation_rep_B
Sample type RNA
Source name macrophages with 0_0 stimulation with LPS
Organism Mus musculus
Characteristics strain: C57Bl/6
cells: bone marrow cells differentiated in M-CSF
Extracted molecule total RNA
Extraction protocol Cells were lysed in TriFast for RNA isolation as recommended by the manufacturer
Label biotin, PE
Label protocol Affymetrix standard procedure
Hybridization protocol Affymetrix standard procedure
Scan protocol Affymetrix standard procedure
Description Cells were either prestimulated (1_X) or not (0_X) for 18h with 100 ng/ml LPS. Media was exchanged and after 2h rest, cells either restimulated (X_1) or not (X_0) with again 100 ng/ml LPS. This resulted in 4 experimental conditions: 0_0, 0_1, 1_0 and 1_1. There were 3 biological replicates done, resulting in 12 arrays altogether.
Data processing rma background correction and normalization. Linear values.
Submission date Jul 29, 2007
Last update date Aug 02, 2019
Contact name Joerg Mages
Organization name Technical University Munich
Department Institute of Medical Microbiology, Immunology and Hygiene
Street address Trogerstrasse 30
City Munich
ZIP/Postal code 81675
Country Germany
Platform ID GPL1261
Series (1)
GSE8621 LPS tolerance in macrophages
Reanalyzed by GSE119085
Reanalyzed by GSE135324

Data table header descriptions
VALUE rma normalized value (linear scale)

Data table
1415670_at 732.18 P
1415671_at 3081.84 P
1415672_at 2741.4 P
1415673_at 219.56 P
1415674_a_at 1007.94 P
1415675_at 505.81 P
1415676_a_at 2574.72 P
1415677_at 688.52 P
1415678_at 1467.24 P
1415679_at 2899.88 P
1415680_at 937.61 P
1415681_at 1031.67 P
1415682_at 351.09 P
1415683_at 2264.74 P
1415684_at 960.28 P
1415685_at 444.62 P
1415686_at 2095.23 P
1415687_a_at 10891.72 P
1415688_at 1123 P
1415689_s_at 507.64 P

Total number of rows: 45101

Table truncated, full table size 866 Kbytes.

Supplementary file Size Download File type/resource
GSM213694.CEL.gz 6.0 Mb (ftp)(http) CEL
Processed data included within Sample table
Raw data included within Sample table
Raw data provided as supplementary file

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