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Sample GSM213695 Query DataSets for GSM213695
Status Public on Aug 10, 2007
Title no_pre_no_restimulation_rep_C
Sample type RNA
 
Source name macrophages with 0_0 stimulation with LPS
Organism Mus musculus
Characteristics strain: C57Bl/6
cells: bone marrow cells differentiated in M-CSF
Extracted molecule total RNA
Extraction protocol Cells were lysed in TriFast for RNA isolation as recommended by the manufacturer
Label biotin, PE
Label protocol Affymetrix standard procedure
 
Hybridization protocol Affymetrix standard procedure
Scan protocol Affymetrix standard procedure
Description Cells were either prestimulated (1_X) or not (0_X) for 18h with 100 ng/ml LPS. Media was exchanged and after 2h rest, cells either restimulated (X_1) or not (X_0) with again 100 ng/ml LPS. This resulted in 4 experimental conditions: 0_0, 0_1, 1_0 and 1_1. There were 3 biological replicates done, resulting in 12 arrays altogether.
Data processing rma background correction and normalization. Linear values.
 
Submission date Jul 29, 2007
Last update date Aug 02, 2019
Contact name Joerg Mages
E-mail(s) mages@lrz.tum.de
Organization name Technical University Munich
Department Institute of Medical Microbiology, Immunology and Hygiene
Street address Trogerstrasse 30
City Munich
ZIP/Postal code 81675
Country Germany
 
Platform ID GPL1261
Series (1)
GSE8621 LPS tolerance in macrophages
Relations
Reanalyzed by GSE119085
Reanalyzed by GSE135324

Data table header descriptions
ID_REF
VALUE rma normalized value (linear scale)
ABS_CALL MAS5 call

Data table
ID_REF VALUE ABS_CALL
1415670_at 638.44 P
1415671_at 3337.38 P
1415672_at 2846.74 P
1415673_at 197.46 P
1415674_a_at 946.9 P
1415675_at 505.56 P
1415676_a_at 2559.51 P
1415677_at 668.11 P
1415678_at 1543.72 P
1415679_at 2851.25 P
1415680_at 846.46 P
1415681_at 941.72 P
1415682_at 357.29 P
1415683_at 2730.13 P
1415684_at 1006.32 P
1415685_at 473.69 P
1415686_at 2264.76 P
1415687_a_at 10797.51 P
1415688_at 1086.16 P
1415689_s_at 514.97 P

Total number of rows: 45101

Table truncated, full table size 865 Kbytes.




Supplementary file Size Download File type/resource
GSM213695.CEL.gz 5.9 Mb (ftp)(http) CEL
Processed data included within Sample table
Raw data provided as supplementary file

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