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Sample GSM213696 Query DataSets for GSM213696
Status Public on Aug 10, 2007
Title no_pre_LPS_restimulation_rep_A
Sample type RNA
 
Source name macrophages with 0_1 stimulation with LPS
Organism Mus musculus
Characteristics strain: C57Bl/6
cells: bone marrow cells differentiated in M-CSF
Extracted molecule total RNA
Extraction protocol Cells were lysed in TriFast for RNA isolation as recommended by the manufacturer
Label biotin, PE
Label protocol Affymetrix standard procedure
 
Hybridization protocol Affymetrix standard procedure
Scan protocol Affymetrix standard procedure
Description Cells were either prestimulated (1_X) or not (0_X) for 18h with 100 ng/ml LPS. Media was exchanged and after 2h rest, cells either restimulated (X_1) or not (X_0) with again 100 ng/ml LPS. This resulted in 4 experimental conditions: 0_0, 0_1, 1_0 and 1_1. There were 3 biological replicates done, resulting in 12 arrays altogether.
Data processing rma background correction and normalization. Linear values.
 
Submission date Jul 29, 2007
Last update date Aug 02, 2019
Contact name Joerg Mages
E-mail(s) mages@lrz.tum.de
Organization name Technical University Munich
Department Institute of Medical Microbiology, Immunology and Hygiene
Street address Trogerstrasse 30
City Munich
ZIP/Postal code 81675
Country Germany
 
Platform ID GPL1261
Series (1)
GSE8621 LPS tolerance in macrophages
Relations
Reanalyzed by GSE119085
Reanalyzed by GSE135324

Data table header descriptions
ID_REF
VALUE rma normalized value (linear scale)
ABS_CALL MAS5 call

Data table
ID_REF VALUE ABS_CALL
1415670_at 752.65 P
1415671_at 2000.6 P
1415672_at 2242.44 P
1415673_at 69.15 P
1415674_a_at 1178.91 P
1415675_at 503.67 P
1415676_a_at 2861.25 P
1415677_at 449.36 P
1415678_at 1554.4 P
1415679_at 2332.81 P
1415680_at 576.74 P
1415681_at 770.64 P
1415682_at 141.3 P
1415683_at 2457.22 P
1415684_at 579.54 P
1415685_at 235.38 P
1415686_at 1353.4 P
1415687_a_at 8140.86 P
1415688_at 1130.79 P
1415689_s_at 257.96 P

Total number of rows: 45101

Table truncated, full table size 865 Kbytes.




Supplementary file Size Download File type/resource
GSM213696.CEL.gz 6.0 Mb (ftp)(http) CEL
Processed data included within Sample table
Raw data provided as supplementary file

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