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Sample GSM213699 Query DataSets for GSM213699
Status Public on Aug 10, 2007
Title LPS_pre_no_restimulation_rep_A
Sample type RNA
 
Source name macrophages with 1_0 stimulation with LPS
Organism Mus musculus
Characteristics strain: C57Bl/6
cells: bone marrow cells differentiated in M-CSF
Extracted molecule total RNA
Extraction protocol Cells were lysed in TriFast for RNA isolation as recommended by the manufacturer
Label biotin, PE
Label protocol Affymetrix standard procedure
 
Hybridization protocol Affymetrix standard procedure
Scan protocol Affymetrix standard procedure
Description Cells were either prestimulated (1_X) or not (0_X) for 18h with 100 ng/ml LPS. Media was exchanged and after 2h rest, cells either restimulated (X_1) or not (X_0) with again 100 ng/ml LPS. This resulted in 4 experimental conditions: 0_0, 0_1, 1_0 and 1_1. There were 3 biological replicates done, resulting in 12 arrays altogether.
Data processing rma background correction and normalization. Linear values.
 
Submission date Jul 29, 2007
Last update date Aug 02, 2019
Contact name Joerg Mages
E-mail(s) mages@lrz.tum.de
Organization name Technical University Munich
Department Institute of Medical Microbiology, Immunology and Hygiene
Street address Trogerstrasse 30
City Munich
ZIP/Postal code 81675
Country Germany
 
Platform ID GPL1261
Series (1)
GSE8621 LPS tolerance in macrophages
Relations
Reanalyzed by GSE119085
Reanalyzed by GSE135324

Data table header descriptions
ID_REF
VALUE rma normalized value (linear scale)
ABS_CALL MAS5 call

Data table
ID_REF VALUE ABS_CALL
1415670_at 726.2 P
1415671_at 2977.46 P
1415672_at 2645.6 P
1415673_at 179.95 P
1415674_a_at 764.25 P
1415675_at 519.43 P
1415676_a_at 2705.43 P
1415677_at 1409.52 P
1415678_at 1103.12 P
1415679_at 2687.04 P
1415680_at 906.72 P
1415681_at 928.76 P
1415682_at 359.92 P
1415683_at 2603.52 P
1415684_at 1051.53 P
1415685_at 454.77 P
1415686_at 2033.44 P
1415687_a_at 10609.36 P
1415688_at 847.73 P
1415689_s_at 426.8 P

Total number of rows: 45101

Table truncated, full table size 865 Kbytes.




Supplementary file Size Download File type/resource
GSM213699.CEL.gz 6.1 Mb (ftp)(http) CEL
Processed data included within Sample table
Raw data provided as supplementary file

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