|
Status |
Public on Aug 10, 2007 |
Title |
LPS_pre_no_restimulation_rep_B |
Sample type |
RNA |
|
|
Source name |
macrophages with 1_0 stimulation with LPS
|
Organism |
Mus musculus |
Characteristics |
strain: C57Bl/6 cells: bone marrow cells differentiated in M-CSF
|
Extracted molecule |
total RNA |
Extraction protocol |
Cells were lysed in TriFast for RNA isolation as recommended by the manufacturer
|
Label |
biotin, PE
|
Label protocol |
Affymetrix standard procedure
|
|
|
Hybridization protocol |
Affymetrix standard procedure
|
Scan protocol |
Affymetrix standard procedure
|
Description |
Cells were either prestimulated (1_X) or not (0_X) for 18h with 100 ng/ml LPS. Media was exchanged and after 2h rest, cells either restimulated (X_1) or not (X_0) with again 100 ng/ml LPS. This resulted in 4 experimental conditions: 0_0, 0_1, 1_0 and 1_1. There were 3 biological replicates done, resulting in 12 arrays altogether.
|
Data processing |
rma background correction and normalization. Linear values.
|
|
|
Submission date |
Jul 29, 2007 |
Last update date |
Aug 02, 2019 |
Contact name |
Joerg Mages |
E-mail(s) |
mages@lrz.tum.de
|
Organization name |
Technical University Munich
|
Department |
Institute of Medical Microbiology, Immunology and Hygiene
|
Street address |
Trogerstrasse 30
|
City |
Munich |
ZIP/Postal code |
81675 |
Country |
Germany |
|
|
Platform ID |
GPL1261 |
Series (1) |
GSE8621 |
LPS tolerance in macrophages |
|
Relations |
Reanalyzed by |
GSE119085 |
Reanalyzed by |
GSE135324 |