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Sample GSM213701 Query DataSets for GSM213701
Status Public on Aug 10, 2007
Title LPS_pre_no_restimulation_rep_C
Sample type RNA
 
Source name macrophages with 1_0 stimulation with LPS
Organism Mus musculus
Characteristics strain: C57Bl/6
cells: bone marrow cells differentiated in M-CSF
Extracted molecule total RNA
Extraction protocol Cells were lysed in TriFast for RNA isolation as recommended by the manufacturer
Label biotin, PE
Label protocol Affymetrix standard procedure
 
Hybridization protocol Affymetrix standard procedure
Scan protocol Affymetrix standard procedure
Description Cells were either prestimulated (1_X) or not (0_X) for 18h with 100 ng/ml LPS. Media was exchanged and after 2h rest, cells either restimulated (X_1) or not (X_0) with again 100 ng/ml LPS. This resulted in 4 experimental conditions: 0_0, 0_1, 1_0 and 1_1. There were 3 biological replicates done, resulting in 12 arrays altogether.
Data processing rma background correction and normalization. Linear values.
 
Submission date Jul 29, 2007
Last update date Aug 02, 2019
Contact name Joerg Mages
E-mail(s) mages@lrz.tum.de
Organization name Technical University Munich
Department Institute of Medical Microbiology, Immunology and Hygiene
Street address Trogerstrasse 30
City Munich
ZIP/Postal code 81675
Country Germany
 
Platform ID GPL1261
Series (1)
GSE8621 LPS tolerance in macrophages
Relations
Reanalyzed by GSE119085
Reanalyzed by GSE135324

Data table header descriptions
ID_REF
VALUE rma normalized value (linear scale)
ABS_CALL MAS5 call

Data table
ID_REF VALUE ABS_CALL
1415670_at 649.01 P
1415671_at 3159.32 P
1415672_at 2770.91 P
1415673_at 182.5 P
1415674_a_at 951.19 P
1415675_at 586.5 P
1415676_a_at 2852.87 P
1415677_at 1096.34 P
1415678_at 1214.79 P
1415679_at 3132.56 P
1415680_at 884.01 P
1415681_at 990.7 P
1415682_at 340.95 P
1415683_at 2399.14 P
1415684_at 1171.45 P
1415685_at 415.41 P
1415686_at 2178.88 P
1415687_a_at 9790.33 P
1415688_at 918.37 P
1415689_s_at 454.78 P

Total number of rows: 45101

Table truncated, full table size 865 Kbytes.




Supplementary file Size Download File type/resource
GSM213701.CEL.gz 6.0 Mb (ftp)(http) CEL
Processed data included within Sample table
Raw data provided as supplementary file

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