|
| Status |
Public on Aug 10, 2007 |
| Title |
LPS_pre_LPS_restimulation_rep_B |
| Sample type |
RNA |
| |
|
| Source name |
macrophages with 1_1 stimulation with LPS
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57Bl/6
cells: bone marrow cells differentiated in M-CSF
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were lysed in TriFast for RNA isolation as recommended by the manufacturer
|
| Label |
biotin, PE
|
| Label protocol |
Affymetrix standard procedure
|
| |
|
| Hybridization protocol |
Affymetrix standard procedure
|
| Scan protocol |
Affymetrix standard procedure
|
| Description |
Cells were either prestimulated (1_X) or not (0_X) for 18h with 100 ng/ml LPS. Media was exchanged and after 2h rest, cells either restimulated (X_1) or not (X_0) with again 100 ng/ml LPS. This resulted in 4 experimental conditions: 0_0, 0_1, 1_0 and 1_1. There were 3 biological replicates done, resulting in 12 arrays altogether.
|
| Data processing |
rma background correction and normalization. Linear values.
|
| |
|
| Submission date |
Jul 29, 2007 |
| Last update date |
Aug 02, 2019 |
| Contact name |
Joerg Mages |
| E-mail(s) |
mages@lrz.tum.de
|
| Organization name |
Technical University Munich
|
| Department |
Institute of Medical Microbiology, Immunology and Hygiene
|
| Street address |
Trogerstrasse 30
|
| City |
Munich |
| ZIP/Postal code |
81675 |
| Country |
Germany |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE8621 |
LPS tolerance in macrophages |
|
| Relations |
| Reanalyzed by |
GSE119085 |
| Reanalyzed by |
GSE135324 |
