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Sample GSM213703 Query DataSets for GSM213703
Status Public on Aug 10, 2007
Title LPS_pre_LPS_restimulation_rep_B
Sample type RNA
Source name macrophages with 1_1 stimulation with LPS
Organism Mus musculus
Characteristics strain: C57Bl/6
cells: bone marrow cells differentiated in M-CSF
Extracted molecule total RNA
Extraction protocol Cells were lysed in TriFast for RNA isolation as recommended by the manufacturer
Label biotin, PE
Label protocol Affymetrix standard procedure
Hybridization protocol Affymetrix standard procedure
Scan protocol Affymetrix standard procedure
Description Cells were either prestimulated (1_X) or not (0_X) for 18h with 100 ng/ml LPS. Media was exchanged and after 2h rest, cells either restimulated (X_1) or not (X_0) with again 100 ng/ml LPS. This resulted in 4 experimental conditions: 0_0, 0_1, 1_0 and 1_1. There were 3 biological replicates done, resulting in 12 arrays altogether.
Data processing rma background correction and normalization. Linear values.
Submission date Jul 29, 2007
Last update date Aug 02, 2019
Contact name Joerg Mages
Organization name Technical University Munich
Department Institute of Medical Microbiology, Immunology and Hygiene
Street address Trogerstrasse 30
City Munich
ZIP/Postal code 81675
Country Germany
Platform ID GPL1261
Series (1)
GSE8621 LPS tolerance in macrophages
Reanalyzed by GSE119085
Reanalyzed by GSE135324

Data table header descriptions
VALUE rma normalized value (linear scale)

Data table
1415670_at 826.2 P
1415671_at 3168.4 P
1415672_at 2540.91 P
1415673_at 256.41 P
1415674_a_at 1202.5 P
1415675_at 544.72 P
1415676_a_at 4583.94 P
1415677_at 1044.55 P
1415678_at 1189.82 P
1415679_at 2703.67 P
1415680_at 926.95 P
1415681_at 896.45 P
1415682_at 288.19 P
1415683_at 2269.54 P
1415684_at 1087.03 P
1415685_at 423.73 P
1415686_at 1861.95 P
1415687_a_at 9540.92 P
1415688_at 769.66 P
1415689_s_at 344.22 P

Total number of rows: 45101

Table truncated, full table size 865 Kbytes.

Supplementary file Size Download File type/resource
GSM213703.CEL.gz 6.0 Mb (ftp)(http) CEL
Processed data included within Sample table
Raw data provided as supplementary file

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