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Sample GSM213704 Query DataSets for GSM213704
Status Public on Aug 10, 2007
Title LPS_pre_LPS_restimulation_rep_C
Sample type RNA
 
Source name macrophages with 1_1 stimulation with LPS
Organism Mus musculus
Characteristics strain: C57Bl/6
cells: bone marrow cells differentiated in M-CSF
Extracted molecule total RNA
Extraction protocol Cells were lysed in TriFast for RNA isolation as recommended by the manufacturer
Label biotin, PE
Label protocol Affymetrix standard procedure
 
Hybridization protocol Affymetrix standard procedure
Scan protocol Affymetrix standard procedure
Description Cells were either prestimulated (1_X) or not (0_X) for 18h with 100 ng/ml LPS. Media was exchanged and after 2h rest, cells either restimulated (X_1) or not (X_0) with again 100 ng/ml LPS. This resulted in 4 experimental conditions: 0_0, 0_1, 1_0 and 1_1. There were 3 biological replicates done, resulting in 12 arrays altogether.
Data processing rma background correction and normalization. Linear values.
 
Submission date Jul 29, 2007
Last update date Aug 02, 2019
Contact name Joerg Mages
E-mail(s) mages@lrz.tum.de
Organization name Technical University Munich
Department Institute of Medical Microbiology, Immunology and Hygiene
Street address Trogerstrasse 30
City Munich
ZIP/Postal code 81675
Country Germany
 
Platform ID GPL1261
Series (1)
GSE8621 LPS tolerance in macrophages
Relations
Reanalyzed by GSE119085
Reanalyzed by GSE135324

Data table header descriptions
ID_REF
VALUE rma normalized value (linear scale)
ABS_CALL MAS5 call

Data table
ID_REF VALUE ABS_CALL
1415670_at 411.94 P
1415671_at 2478.54 P
1415672_at 1783.53 P
1415673_at 57.88 P
1415674_a_at 958.61 P
1415675_at 410.79 P
1415676_a_at 2621.29 P
1415677_at 657.63 P
1415678_at 1107.03 P
1415679_at 2271.53 P
1415680_at 761.09 P
1415681_at 661.03 P
1415682_at 155.6 P
1415683_at 2274.59 P
1415684_at 553.51 P
1415685_at 187.75 P
1415686_at 1585.75 P
1415687_a_at 7833.21 P
1415688_at 705.41 P
1415689_s_at 164.61 P

Total number of rows: 45101

Table truncated, full table size 862 Kbytes.




Supplementary file Size Download File type/resource
GSM213704.CEL.gz 6.4 Mb (ftp)(http) CEL
Processed data included within Sample table
Raw data provided as supplementary file

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