|
| Status |
Public on May 31, 2018 |
| Title |
14Z66P5 |
| Sample type |
SRA |
| |
|
| Source name |
Mesenchymal stem cells
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Skin-derived MSC
passages: 5
status: Normal
|
| Growth protocol |
Skin derived mesenchymal stem cells were cultured in DMDM/F12 medium and adherent cultured
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from each sample was used to prepare the circRNA sequencing library
5ug total RNA were pretreated to enrich circRNA using CircRNA Enrichment Kit (Cloud-seq Inc, USA). RNA libraries were constructed by using pretreated RNAs with TruSeq Stranded Total RNA Library Prep Kit (Illumina, San Diego, CA, USA) according to the manufacturer’s instructions. Libraries were controlled for quality and quantified using the BioAnalyzer 2100 system (Agilent Technologies, Inc., USA). The libraries were denatured as single-stranded DNA molecules, captured on Illumina flow cells, amplified in situ as clusters and finally sequenced for 150 cycles on Illumina HiSeq Sequencer according to the manufacturer’s instructions.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 4000 |
| |
|
| Data processing |
The reads were aligned to the reference genome/transcriptome with BWA-MEM software and circRNAs were detected and annotated with CIRI software
Genome_build: hg19
Supplementary_files_format_and_content: circRNA expression profiles
|
| |
|
| Submission date |
May 04, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Ruifeng Liu |
| E-mail(s) |
luyaoliu@126.com
|
| Phone |
86-351-5656076
|
| Organization name |
Taiyuan City Centre Hospital
|
| Department |
Institute of Dermatology
|
| Lab |
Shanxi Provincial Key Laboratory of immunological skin diseases
|
| Street address |
No. 1 Dong San Dao Xiang
|
| City |
Taiyuan |
| State/province |
Shanxi |
| ZIP/Postal code |
030009 |
| Country |
China |
| |
|
| Platform ID |
GPL20301 |
| Series (1) |
|
| Relations |
| BioSample |
SAMN04942732 |
| SRA |
SRX1742627 |
