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Sample GSM214783 Query DataSets for GSM214783
Status Public on Aug 20, 2007
Title 24h DC6 HS
Sample type RNA
Source name Monocyte derived dendritic cells
Organism Homo sapiens
Characteristics tissue: freshly isolated human monocyte
gender:male; age: 52 yr; weight: 75 kg
Biomaterial provider Blood Center
Treatment protocol Cells were treated with vehicle (DMSO/ethanol). Ligands or vehicle were added to the cell culture starting from the first day.
Growth protocol Monocytes were cultured for 24 hours with 500 U/ml IL-4 and 800 U/ml GM-CSF. Cells were cultured in RPMI medium containing 10% human AB serum (HS) instead of FBS.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using Trizol Reagent (Invitrogen) and further purified by using the RNeasy kit (Quiagen)
Label biotin
Label protocol cRNA was generated from 5 μg of total RNA by using the SuperScript Choice kit (Invitrogen) and the High Yield RNA transcription labeling kit (Enzo Diagnostics).
Hybridization protocol standard Affymetrix protocol
Scan protocol standard Affymetrix protocol
Description Monocytes (98% CD14+) were obtained from Buffy coats by Ficoll gradient centrifugation and magnetic cell separation using anti-CD14-conjugated microbeads (VarioMACS; Miltenyi Biotec)
Data processing GC-RMA algorithm
Submission date Aug 02, 2007
Last update date Aug 28, 2018
Contact name Istvan Szatmari
Phone 36 52 416-432
Fax 36 52 314-989
Organization name University of Debrecen
Department Department of Biochemistry and Molecular Biology
Lab Nuclear Hormone Receptor
Street address Egyetem tér 1.
City Debrecen
ZIP/Postal code H-4010
Country Hungary
Platform ID GPL570
Series (1)
GSE8658 PPARg regulated gene expression in human dendritic cells
Reanalyzed by GSE64985
Reanalyzed by GSE119087

Data table header descriptions
VALUE Signal

Data table
1007_s_at 36.86
1053_at 165.7
117_at 558
121_at 204.5
1255_g_at 4.74
1294_at 51.22
1316_at 45.02
1320_at 5.884
1405_i_at 47.57
1431_at 7.22
1438_at 4.989
1487_at 197.8
1494_f_at 21.84
1552256_a_at 251.1
1552257_a_at 439.7
1552258_at 48.54
1552261_at 7.584
1552263_at 297.5
1552264_a_at 566.6
1552266_at 10.46

Total number of rows: 54675

Table truncated, full table size 888 Kbytes.

Supplementary file Size Download File type/resource
GSM214783.CEL.gz 4.8 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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