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Sample GSM214788 Query DataSets for GSM214788
Status Public on Aug 20, 2007
Title 24h DC C114R RSG
Sample type RNA
Source name Monocyte derived dendritic cells
Organism Homo sapiens
Characteristics tissue: freshly isolated human monocyte
gender:female; age: 41 yr; BMI: 30
Further information: Agostini at al. 2006 Cell Metabolism, 4 303-311
Biomaterial provider Blood Bank
Treatment protocol Cells were treated with 1 uM rosiglitazone (RSG). Ligands or vehicle were added to the cell culture starting from the first day.
Growth protocol Monocytes were cultured for 24 hours with 500 U/ml IL-4 and 800 U/ml GM-CSF.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using Trizol Reagent (Invitrogen).
Label biotin
Label protocol cRNA was generated from 5 μg of total RNA by using the SuperScript Choice kit (Invitrogen) and the High Yield RNA transcription labeling kit (Enzo Diagnostics).
Hybridization protocol standard Affymetrix protocol
Scan protocol standard Affymetrix protocol
Description Monocytes (98% CD14+) were obtained from Buffy coats by Ficoll gradient centrifugation and magnetic cell separation using anti-CD14-conjugated microbeads (VarioMACS; Miltenyi Biotec)
Data processing GC-RMA algorithm
Submission date Aug 02, 2007
Last update date Aug 28, 2018
Contact name Istvan Szatmari
Phone 36 52 416-432
Fax 36 52 314-989
Organization name University of Debrecen
Department Department of Biochemistry and Molecular Biology
Lab Nuclear Hormone Receptor
Street address Egyetem tér 1.
City Debrecen
ZIP/Postal code H-4010
Country Hungary
Platform ID GPL570
Series (1)
GSE8658 PPARg regulated gene expression in human dendritic cells
Reanalyzed by GSE64985
Reanalyzed by GSE119087

Data table header descriptions
VALUE Signal

Data table
1007_s_at 27.11
1053_at 253.1
117_at 227.2
121_at 219.2
1255_g_at 4.55
1294_at 97.39
1316_at 32.82
1320_at 6.132
1405_i_at 230.5
1431_at 7.551
1438_at 5.011
1487_at 509.2
1494_f_at 20.33
1552256_a_at 94.06
1552257_a_at 608
1552258_at 46.36
1552261_at 24.59
1552263_at 376.1
1552264_a_at 565.6
1552266_at 12.34

Total number of rows: 54675

Table truncated, full table size 888 Kbytes.

Supplementary file Size Download File type/resource
GSM214788.CEL.gz 8.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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