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Sample GSM214796 Query DataSets for GSM214796
Status Public on Aug 20, 2007
Title 5d DC3 RSG
Sample type RNA
 
Source name Monocyte derived dendritic cells
Organism Homo sapiens
Characteristics tissue: freshly isolated human monocyte
gender:male; age: 41 yr; weight: 92 kg
Biomaterial provider Blood Center
Treatment protocol Cells were treated with 2.5 uM rosiglitazone (RSG). Ligands or vehicle were added to the cell culture starting from the first day.
Growth protocol Monocytes were cultured for 5 days with 500 U/ml IL-4 and 800 U/ml GM-CSF. Cytokine treatment was repeated at day 3.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using Trizol Reagent (Invitrogen) and further purified by using the RNeasy kit (Quiagen).
Label biotin
Label protocol cRNA was generated from 5 μg of total RNA by using the SuperScript Choice kit (Invitrogen) and the High Yield RNA transcription labeling kit (Enzo Diagnostics).
 
Hybridization protocol standard Affymetrix protocol
Scan protocol standard Affymetrix protocol
Description Monocytes (98% CD14+) were obtained from Buffy coats by Ficoll gradient centrifugation and magnetic cell separation using anti-CD14-conjugated microbeads (VarioMACS; Miltenyi Biotec)
Data processing GC-RMA algorithm
 
Submission date Aug 02, 2007
Last update date Aug 28, 2018
Contact name Istvan Szatmari
E-mail(s) szatmari@indi.biochem.dote.hu
Phone 36 52 416-432
Fax 36 52 314-989
Organization name University of Debrecen
Department Department of Biochemistry and Molecular Biology
Lab Nuclear Hormone Receptor
Street address Egyetem tér 1.
City Debrecen
ZIP/Postal code H-4010
Country Hungary
 
Platform ID GPL570
Series (1)
GSE8658 PPARg regulated gene expression in human dendritic cells
Relations
Reanalyzed by GSE64985
Reanalyzed by GSE119087

Data table header descriptions
ID_REF
VALUE Signal

Data table
ID_REF VALUE
1007_s_at 14.73
1053_at 103.4
117_at 59.96
121_at 259.1
1255_g_at 327.1
1294_at 121.9
1316_at 31.68
1320_at 7.765
1405_i_at 990.1
1431_at 9.176
1438_at 5.584
1487_at 139.8
1494_f_at 28.97
1552256_a_at 1273
1552257_a_at 303.8
1552258_at 25.02
1552261_at 18.61
1552263_at 233.6
1552264_a_at 453.9
1552266_at 13.39

Total number of rows: 54675

Table truncated, full table size 888 Kbytes.




Supplementary file Size Download File type/resource
GSM214796.CEL.gz 4.6 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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