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Sample GSM214800 Query DataSets for GSM214800
Status Public on Aug 20, 2007
Title 5d DC4 RSG
Sample type RNA
 
Source name Monocyte derived dendritic cells
Organism Homo sapiens
Characteristics tissue: freshly isolated human monocyte
gender:male; age: 21 yr; weight: 110 kg
Biomaterial provider Blood Center
Treatment protocol Cells were treated with 2.5 uM rosiglitazone (RSG). Ligands or vehicle were added to the cell culture starting from the first day.
Growth protocol Monocytes were cultured for 5 days with 500 U/ml IL-4 and 800 U/ml GM-CSF. Cytokine treatment was repeated at day 3.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using Trizol Reagent (Invitrogen) and further purified by using the RNeasy kit (Quiagen).
Label biotin
Label protocol cRNA was generated from 5 μg of total RNA by using the SuperScript Choice kit (Invitrogen) and the High Yield RNA transcription labeling kit (Enzo Diagnostics).
 
Hybridization protocol standard Affymetrix protocol
Scan protocol standard Affymetrix protocol
Description Monocytes (98% CD14+) were obtained from Buffy coats by Ficoll gradient centrifugation and magnetic cell separation using anti-CD14-conjugated microbeads (VarioMACS; Miltenyi Biotec)
Data processing GC-RMA algorithm
 
Submission date Aug 02, 2007
Last update date Aug 28, 2018
Contact name Istvan Szatmari
E-mail(s) szatmari@indi.biochem.dote.hu
Phone 36 52 416-432
Fax 36 52 314-989
Organization name University of Debrecen
Department Department of Biochemistry and Molecular Biology
Lab Nuclear Hormone Receptor
Street address Egyetem tér 1.
City Debrecen
ZIP/Postal code H-4010
Country Hungary
 
Platform ID GPL570
Series (1)
GSE8658 PPARg regulated gene expression in human dendritic cells
Relations
Reanalyzed by GSE64985
Reanalyzed by GSE119087

Data table header descriptions
ID_REF
VALUE Signal

Data table
ID_REF VALUE
1007_s_at 11.91
1053_at 87.07
117_at 161.5
121_at 327.5
1255_g_at 412
1294_at 164.9
1316_at 25.38
1320_at 6.076
1405_i_at 1018
1431_at 7.433
1438_at 5.244
1487_at 151.3
1494_f_at 20.12
1552256_a_at 968.6
1552257_a_at 378.2
1552258_at 57.85
1552261_at 19.09
1552263_at 246.1
1552264_a_at 616.8
1552266_at 11.55

Total number of rows: 54675

Table truncated, full table size 888 Kbytes.




Supplementary file Size Download File type/resource
GSM214800.CEL.gz 4.6 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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