|
| Status |
Public on Aug 20, 2007 |
| Title |
5d DC5 |
| Sample type |
RNA |
| |
|
| Source name |
Monocyte derived dendritic cells
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: freshly isolated human monocyte
gender:male; age: 18 yr; weight: 82 kg
|
| Biomaterial provider |
Blood Center
|
| Treatment protocol |
Cells were treated with vehicle (DMSO/ethanol). Ligands or vehicle were added to the cell culture starting from the first day.
|
| Growth protocol |
Monocytes were cultured for 5 days with 500 U/ml IL-4 and 800 U/ml GM-CSF. Cytokine treatment was repeated at day 3.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using Trizol Reagent (Invitrogen) and further purified by using the RNeasy kit (Quiagen).
|
| Label |
biotin
|
| Label protocol |
cRNA was generated from 5 μg of total RNA by using the SuperScript Choice kit (Invitrogen) and the High Yield RNA transcription labeling kit (Enzo Diagnostics).
|
| |
|
| Hybridization protocol |
standard Affymetrix protocol
|
| Scan protocol |
standard Affymetrix protocol
|
| Description |
Monocytes (98% CD14+) were obtained from Buffy coats by Ficoll gradient centrifugation and magnetic cell separation using anti-CD14-conjugated microbeads (VarioMACS; Miltenyi Biotec)
|
| Data processing |
GC-RMA algorithm
|
| |
|
| Submission date |
Aug 02, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Istvan Szatmari |
| E-mail(s) |
szatmari@indi.biochem.dote.hu
|
| Phone |
36 52 416-432
|
| Fax |
36 52 314-989
|
| Organization name |
University of Debrecen
|
| Department |
Department of Biochemistry and Molecular Biology
|
| Lab |
Nuclear Hormone Receptor
|
| Street address |
Egyetem tér 1.
|
| City |
Debrecen |
| ZIP/Postal code |
H-4010 |
| Country |
Hungary |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE8658 |
PPARg regulated gene expression in human dendritic cells |
|
| Relations |
| Reanalyzed by |
GSE64985 |
| Reanalyzed by |
GSE119087 |
