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Sample GSM214805 Query DataSets for GSM214805
Status Public on Aug 20, 2007
Title 5d DC6 HS
Sample type RNA
 
Source name Monocyte derived dendritic cells
Organism Homo sapiens
Characteristics tissue: freshly isolated human monocyte
gender:male; age: 18 yr; weight: 73 kg
Biomaterial provider Blood Center
Treatment protocol Cells were treated with vehicle (DMSO/ethanol). Ligands or vehicle were added to the cell culture starting from the first day.
Growth protocol Monocytes were cultured for 5 days with 500 U/ml IL-4 and 800 U/ml GM-CSF. Cytokine treatment was repeated at day 3. Cells were cultured in RPMI medium containing 10% human AB serum (HS) instead of FBS.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using Trizol Reagent (Invitrogen) and further purified by using the RNeasy kit (Quiagen).
Label biotin
Label protocol cRNA was generated from 5 μg of total RNA by using the SuperScript Choice kit (Invitrogen) and the High Yield RNA transcription labeling kit (Enzo Diagnostics).
 
Hybridization protocol standard Affymetrix protocol
Scan protocol standard Affymetrix protocol
Description Monocytes (98% CD14+) were obtained from Buffy coats by Ficoll gradient centrifugation and magnetic cell separation using anti-CD14-conjugated microbeads (VarioMACS; Miltenyi Biotec)
Data processing GC-RMA algorithm
 
Submission date Aug 02, 2007
Last update date Aug 28, 2018
Contact name Istvan Szatmari
E-mail(s) szatmari@indi.biochem.dote.hu
Phone 36 52 416-432
Fax 36 52 314-989
Organization name University of Debrecen
Department Department of Biochemistry and Molecular Biology
Lab Nuclear Hormone Receptor
Street address Egyetem tér 1.
City Debrecen
ZIP/Postal code H-4010
Country Hungary
 
Platform ID GPL570
Series (1)
GSE8658 PPARg regulated gene expression in human dendritic cells
Relations
Reanalyzed by GSE64985
Reanalyzed by GSE119087

Data table header descriptions
ID_REF
VALUE Signal

Data table
ID_REF VALUE
1007_s_at 34.03
1053_at 170.5
117_at 1011
121_at 153.2
1255_g_at 98.04
1294_at 176.3
1316_at 23.1
1320_at 5.693
1405_i_at 199.9
1431_at 7.037
1438_at 5.583
1487_at 129.3
1494_f_at 25.12
1552256_a_at 175.9
1552257_a_at 316.3
1552258_at 93.85
1552261_at 7.649
1552263_at 230.2
1552264_a_at 576.1
1552266_at 9.889

Total number of rows: 54675

Table truncated, full table size 888 Kbytes.




Supplementary file Size Download File type/resource
GSM214805.CEL.gz 5.0 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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