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Sample GSM215270 Query DataSets for GSM215270
Status Public on Jan 11, 2008
Title 3T3-L1 adipocyte tissue culture, LA fed, T3, biological rep4
Sample type RNA
 
Source name 3T3-L1 adipocyte tissue culture, LA, 12 hours
Organism Mus musculus
Characteristics cell line:3T3-L1
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol (reaction at 37C for 16h) from 15 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C, refer to GeneChip Expression Analysis Technical Manual (www.affymetrix.com). GeneChips were washed and stained with streptavidin-phycoerythrin conjugate on an Affymetrix Fluidics Station 450
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G; Pixel size: 1.56 um; Filter: 570 nm
Description mouse 3T3-L1 RNA for each time point was isolated from control (LA) and treatment (CLA) samples
Data processing The data was analysed using Rosetta Resolver 6.0, creating ratio-experiments for each timepoint using control reps as one pool, and treatment reps as the other pool. Resulting logratios and p-values are calculated using Rosetta's proprietary ratio error model.
 
Submission date Aug 03, 2007
Last update date Aug 28, 2018
Contact name Jean-Jack M. Riethoven
E-mail(s) jeanjack@unl.edu
Organization name University of Nebraska-Lincoln
Department Beadle Center for Biotechnology
Lab Bioinformatics Core Research Facility
Street address 1901 Vine Street
City Lincoln
State/province NE
ZIP/Postal code 68588-0665
Country USA
 
Platform ID GPL1261
Series (2)
GSE8681 Gene expression in mouse 3T3-L1 adipocyte tissue culture treated with CLA
GSE11384 Trans-10, cis-12 conjugated linoleic acid activates the integrated stress response pathway in adipocytes
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE Rosetta Resolver proprietary within-array normalised intensities
DETECTION P-VALUE

Data table
ID_REF VALUE DETECTION P-VALUE
1455571_x_at 691.66986 0
1455572_x_at 5726.40771 0
1436152_a_at 662.86108 0
1436153_a_at 70.8093 4.81E-12
1455573_at 4.83982 0.28414
1436158_at 177.19411 4.49E-31
1455575_at 94.83517 1.97E-07
1436162_at -6.20829 0.77662
1436165_at 31.20741 0.00023
1455578_x_at 4138.40234 0
1436167_at -5.27635 0.67315
1436170_a_at 3.86626 0.33528
1455579_at -1.03711 0.55044
1436172_at 15.28515 0.03085
1455581_x_at 23.57421 0.0041
1455582_at 10.23568 0.19011
1436176_at 0.07099 0.49653
1436179_a_at 203.03696 9.92E-38
1436180_at 313.76688 0
1436182_at -2.68954 0.61011

Total number of rows: 45101

Table truncated, full table size 1229 Kbytes.




Supplementary file Size Download File type/resource
GSM215270.CEL.gz 3.2 Mb (ftp)(http) CEL
Processed data included within Sample table
Processed data are available on Series record

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