NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM215308 Query DataSets for GSM215308
Status Public on Jan 11, 2008
Title 3T3-L1 preadipocyte tissue culture, CLA treated, T3, biological rep1
Sample type RNA
 
Source name 3T3-L1 preadipocyte tissue culture, CLA, 12 hours
Organism Mus musculus
Characteristics cell line:3T3-L1
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol (reaction at 37C for 16h) from 15 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C, refer to GeneChip Expression Analysis Technical Manual (www.affymetrix.com). GeneChips were washed and stained with streptavidin-phycoerythrin conjugate on an Affymetrix Fluidics Station 450
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G; Pixel size: 1.56 um; Filter: 570 nm
Description 3T3-L1 mouse tissue (preadipocytes) treated with CLA and control samples
Data processing The data was analysed using Rosetta Resolver 6.0, creating ratio-experiments for each timepoint using control reps as one pool, and treatment reps as the other pool. Resulting logratios and p-values are calculated using Rosetta's proprietary ratio error model.
 
Submission date Aug 03, 2007
Last update date Aug 28, 2018
Contact name Jean-Jack M. Riethoven
E-mail(s) jeanjack@unl.edu
Organization name University of Nebraska-Lincoln
Department Beadle Center for Biotechnology
Lab Bioinformatics Core Research Facility
Street address 1901 Vine Street
City Lincoln
State/province NE
ZIP/Postal code 68588-0665
Country USA
 
Platform ID GPL1261
Series (2)
GSE8683 Gene expression in 3T3-L1 mouse tissue (preadipocytes) treated with Trans-10,Cis-12 conjugated linoleic acid(t10c12 CLA)
GSE11384 Trans-10, cis-12 conjugated linoleic acid activates the integrated stress response pathway in adipocytes
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE Rosetta Resolver proprietary within-array normalised intensities
DETECTION P-VALUE

Data table
ID_REF VALUE DETECTION P-VALUE
1455571_x_at 2636.61768 0
1455572_x_at 7576.17529 0
1436152_a_at 1335.43042 0
1436153_a_at 473.76178 0
1455573_at 1.56137 0.43262
1436158_at 120.69044 2.42E-16
1455575_at 42.92465 0.01772
1436162_at -3.99947 0.66539
1436165_at 478.28381 0
1455578_x_at 4529.56445 3.64E-37
1436167_at 53.86163 0.00008
1436170_a_at -4.17141 0.65977
1455579_at -2.76436 0.62092
1436172_at 86.21886 4.35E-13
1455581_x_at 148.01561 9.23E-23
1455582_at 14.16433 0.11508
1436176_at 2.33039 0.39132
1436179_a_at 679.79126 0
1436180_at 1004.66345 0
1436182_at -9.55724 0.82986

Total number of rows: 45101

Table truncated, full table size 1223 Kbytes.




Supplementary file Size Download File type/resource
GSM215308.CEL.gz 3.5 Mb (ftp)(http) CEL
Processed data included within Sample table
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap