|
| Status |
Public on Jun 13, 2008 |
| Title |
20 uM hemin (20B) |
| Sample type |
RNA |
| |
|
| Source name |
20 uM hemin
|
| Organism |
Caenorhabditis elegans |
| Characteristics |
L4 stage
|
| Treatment protocol |
For microarray analysis, synchronized F2 larvae were re-inoculated in mCeHR-2 medium supplemented with 4, 20 or 500 uM hemin and harvested at the late L4 stage for mRNA prep and probe hybridization to Affymetrix C. elegans Whole Genome Expression Arrays.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
standard Affymetrix protocol
|
| Label |
biotin
|
| Label protocol |
standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
standard Affymetrix protocol
|
| Scan protocol |
standard Affymetrix protocol
|
| Description |
no additional information
|
| Data processing |
Microarray data were verified with Microarray Suite 5.0 (Affymetrix) and Robust Multichip Average Method (RMA, R package)
|
| |
|
| Submission date |
Aug 06, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
WeiPing Chen |
| E-mail(s) |
weipingChen@niddk.nih.gov
|
| Phone |
301-496-0175
|
| Organization name |
NIDDK/NIH
|
| Department |
GCL
|
| Lab |
Genomics Core Lab
|
| Street address |
Bldg 8, Room 1A11, NIDDK/NIH
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL200 |
| Series (1) |
| GSE8696 |
Heme homeostasis is regulated by the conserved and concerted functions of HRG-1 proteins |
|
