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Sample GSM215572 Query DataSets for GSM215572
Status Public on Aug 07, 2007
Title Diet Induced Obese rat C1
Sample type RNA
 
Source name epididymal fat
Organism Rattus norvegicus
Characteristics Long Evans, 16-26 weeks, DIO (diet induced obese) group
Treatment protocol Epididymal fat tissues were isolated and placed on ice in the Trizol solution
Growth protocol Animals were maintained on a 12:12-hour light-dark photoperiod (light on 10:00 PM).
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 5 microg of cRNA were hybridized on R230_2 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description Gene expression data from epididymal fat tissues.
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1500.
 
Submission date Aug 06, 2007
Last update date Aug 14, 2011
Contact name Dan Li
E-mail(s) li_shuyu_dan@lilly.com
Phone 317-651-9923
Organization name Eli Lilly & Company
Street address Lilly Corporate Center
City Indianapolis
State/province IN
ZIP/Postal code 46285
Country USA
 
Platform ID GPL1355
Series (1)
GSE8700 Expression data from epididymal fat tissues of diet induced obese rats

Data table header descriptions
ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 3893.9 P 0.00034
AFFX-BioB-M_at 5571.1 P 0.000044
AFFX-BioB-3_at 3357.1 P 0.000044
AFFX-BioC-5_at 9260.3 P 0.00006
AFFX-BioC-3_at 9606.2 P 0.000044
AFFX-BioDn-5_at 17074.9 P 0.000052
AFFX-BioDn-3_at 49041.8 P 0.00006
AFFX-CreX-5_at 127187.4 P 0.000052
AFFX-CreX-3_at 144113.2 P 0.000044
AFFX-DapX-5_at 66.7 A 0.440646
AFFX-DapX-M_at 103.9 A 0.227636
AFFX-DapX-3_at 8.5 A 0.992384
AFFX-LysX-5_at 59.4 A 0.425962
AFFX-LysX-M_at 71.4 A 0.5
AFFX-LysX-3_at 70.2 A 0.262827
AFFX-PheX-5_at 30.5 A 0.824672
AFFX-PheX-M_at 15.9 A 0.760937
AFFX-PheX-3_at 43 A 0.712257
AFFX-ThrX-5_at 16.1 A 0.932322
AFFX-ThrX-M_at 26.4 A 0.737173

Total number of rows: 31099

Table truncated, full table size 851 Kbytes.




Supplementary file Size Download File type/resource
GSM215572.CEL.gz 2.5 Mb (ftp)(http) CEL
Processed data included within Sample table

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