|
| Status |
Public on Aug 07, 2007 |
| Title |
control lean rat A4 |
| Sample type |
RNA |
| |
|
| Source name |
epididymal fat
|
| Organism |
Rattus norvegicus |
| Characteristics |
Long Evans, 16-26 weeks, control lean group
|
| Treatment protocol |
Epididymal fat tissues were isolated and placed on ice in the Trizol solution
|
| Growth protocol |
Animals were maintained on a 12:12-hour light-dark photoperiod (light on 10:00 PM).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 5 microg of cRNA were hybridized on R230_2 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from epididymal fat tissues.
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 1500.
|
| |
|
| Submission date |
Aug 06, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Dan Li |
| E-mail(s) |
li_shuyu_dan@lilly.com
|
| Phone |
317-651-9923
|
| Organization name |
Eli Lilly & Company
|
| Street address |
Lilly Corporate Center
|
| City |
Indianapolis |
| State/province |
IN |
| ZIP/Postal code |
46285 |
| Country |
USA |
| |
|
| Platform ID |
GPL1355 |
| Series (1) |
| GSE8700 |
Expression data from epididymal fat tissues of diet induced obese rats |
|
