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Sample GSM216533 Query DataSets for GSM216533
Status Public on Aug 01, 2008
Title esg_females_2
Sample type RNA
 
Source name whole bodies of 42 day old esg female flies, replicate 2
Organism Drosophila melanogaster
Characteristics whole bodies of 42 day old esg female flies, replicate 2
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's (Affymetrix) instructions
Label Biotinylated cRNA
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 mkg of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description Seven lines homozygous for a single P-element insertion led to an increase in life span. Two replicates of each sex and line were used to identify differences in expression from the isogenic control line. Flies were aged 6 weeks (42 days) and frozen at approximately the same time (10:30 -12:00 AM) for RNA extraction.
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
 
Submission date Aug 08, 2007
Last update date Aug 28, 2018
Contact name Mike Magwire
E-mail(s) mmmagwire@hotmail.com
Phone 919-515-5770
Organization name NCSU
Department Genetics
Lab Mackay
Street address 3501 Gardner Hall
City Raleigh
State/province NC
ZIP/Postal code 27695
Country USA
 
Platform ID GPL1322
Series (1)
GSE8775 Mutations Increasing Drosophila melanogaster Life Span
Relations
Reanalyzed by GSE119084

Data table header descriptions
ID_REF
VALUE signal
ABS_CALL
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 261.625 P 0.000753643
AFFX-BioB-M_at 278.019 P 0.000753643
AFFX-BioB-3_at 140.169 P 0.00141043
AFFX-BioC-5_at 422.24 P 0.000340305
AFFX-BioC-3_at 413.265 P 5.16732e-05
AFFX-BioDn-5_at 1003.37 P 4.42873e-05
AFFX-BioDn-3_at 3022.34 P 8.14279e-05
AFFX-CreX-5_at 6542.07 P 5.16732e-05
AFFX-CreX-3_at 8024.7 P 4.42873e-05
AFFX-DapX-5_at 166.135 P 8.14279e-05
AFFX-DapX-M_at 392.492 P 0.000581214
AFFX-DapX-3_at 997.331 P 4.42873e-05
AFFX-LysX-5_at 29.6324 P 0.00499819
AFFX-LysX-M_at 45.732 A 0.327079
AFFX-LysX-3_at 198.661 P 0.000146581
AFFX-PheX-5_at 50.0469 P 0.00359458
AFFX-PheX-M_at 80.6577 A 0.0726999
AFFX-PheX-3_at 145.088 P 0.000169227
AFFX-ThrX-5_at 39.9877 P 0.026111
AFFX-ThrX-M_at 69.6862 P 0.00227496

Total number of rows: 18952

Table truncated, full table size 581 Kbytes.




Supplementary file Size Download File type/resource
GSM216533.CEL.gz 2.2 Mb (ftp)(http) CEL
GSM216533.CHP.gz 106.4 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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