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Sample GSM217323 Query DataSets for GSM217323
Status Public on Dec 01, 2007
Title Liver_HF_week2
Sample type RNA
Source name Liver, high-fat
Organism Mus musculus
Characteristics C57/BL6J
Female mice
Biomaterial provider Animal Holding Unit, National University Singapore
Treatment protocol Liver samples comprising identical lobes were quickly removed from each mouse. The liver samples were immediately snap-frozen in liquid nitrogen and stored at -80┬░C.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from the frozen liver tissue of each mouse using Trizol reagent (Life Technologies, 15596-018) and purified using RNeasy Mini Kit (Qiagen, 74104), according to manufacturer's protocol.
Label Biotin
Label protocol Biotinylated cRNA were prepared using the Genechip IVT Labelling Kit (Affymetrix, 900182), according to manufacturer's protocol.
Hybridization protocol Following fragmentation, the labeled cRNA were hybridized to the Affymetrix GeneChip® Mouse Genome 430 2.0 chip according to the protocol provided by the manufacturer.
Scan protocol GeneChips were scanned using the Agilent GeneArray Scanner G2500A.
Description Liver tissue from C57BL/6J mice fed on high-fat diets
Data processing The GeneChip Operating Software Version (Affymetrix) was used to analyze the scanned image. The normalization target was set to 500.
Submission date Aug 10, 2007
Last update date Dec 29, 2011
Contact name Mei Yen Chan
Organization name Yong Loo Lin School of Medicine, National University of Singapore
Department Pediatrics
Lab Cardiovascular Genetics
Street address 5 Lower Kent Ridge Road
City Singapore
State/province Singapore
ZIP/Postal code 119074
Country Singapore
Platform ID GPL339
Series (1)
GSE8753 Sequential responses to high-fat feeding in an obese mouse model

Data table header descriptions
VALUE Calculated signal intensity
ABS_CALL Detection
DETECTION P-VALUE P-value that indicates the significance level of the detection call

Data table
1419679_at 3.9 A 0.962402
1424270_at 3.4 A 0.919434
1450295_s_at 12 A 0.850342
1419845_at 2.5 A 0.904785
1422249_s_at 3.8 A 0.828613
1437544_at 4.6 A 0.633789
1418358_at 15.3 A 0.5
1437472_at 6.5 A 0.696289
1423056_at 2.5 A 0.932373
1421061_at 7 A 0.753906
1422044_at 15.8 A 0.466064
1435944_s_at 5.1 A 0.870361
1419937_at 22 A 0.696289
1419763_at 4.4 A 0.665527
1421036_at 7.8 A 0.919434
1421988_at 10 A 0.567627
1415801_at 19.4 A 0.80542
1426153_a_at 8.2 A 0.932373
1427610_at 11.8 A 0.633789
1431214_at 62.3 A 0.149658

Total number of rows: 22690

Table truncated, full table size 620 Kbytes.

Supplementary file Size Download File type/resource
GSM217323_HighFat1w_MOE430A_15_07_04.CEL.gz 3.4 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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