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Sample GSM217342 Query DataSets for GSM217342
Status Public on Dec 01, 2007
Title Liver_HF_week4
Sample type RNA
 
Source name Liver, high-fat
Organism Mus musculus
Characteristics C57/BL6J
Female mice
Biomaterial provider Animal Holding Unit, National University Singapore
Treatment protocol Liver samples comprising identical lobes were quickly removed from each mouse. The liver samples were immediately snap-frozen in liquid nitrogen and stored at -80┬░C.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from the frozen liver tissue of each mouse using Trizol reagent (Life Technologies, 15596-018) and purified using RNeasy Mini Kit (Qiagen, 74104), according to manufacturer's protocol.
Label Biotin
Label protocol Biotinylated cRNA were prepared using the Genechip IVT Labelling Kit (Affymetrix, 900182), according to manufacturer's protocol.
 
Hybridization protocol Following fragmentation, the labeled cRNA were hybridized to the Affymetrix GeneChip® Mouse Genome 430 2.0 chip according to the protocol provided by the manufacturer.
Scan protocol GeneChips were scanned using the Agilent GeneArray Scanner G2500A.
Description Liver tissue from C57BL/6J mice fed on high-fat diets
Data processing The GeneChip Operating Software Version 1.2.0.037 (Affymetrix) was used to analyze the scanned image. The normalization target was set to 500.
 
Submission date Aug 12, 2007
Last update date Aug 28, 2018
Contact name Mei Yen Chan
E-mail(s) m_y_chan3@hotmail.com
Organization name Yong Loo Lin School of Medicine, National University of Singapore
Department Pediatrics
Lab Cardiovascular Genetics
Street address 5 Lower Kent Ridge Road
City Singapore
State/province Singapore
ZIP/Postal code 119074
Country Singapore
 
Platform ID GPL1261
Series (1)
GSE8753 Sequential responses to high-fat feeding in an obese mouse model
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE Signal intensity
ABS_CALL Detection
DETECTION P-VALUE p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
1419679_at 32.3 A 0.334473
1424270_at 29.7 A 0.80542
1450295_s_at 85.4 A 0.091553
1419845_at 24.9 A 0.432373
1422249_s_at 47.9 P 0.030273
1437544_at 74.1 A 0.067627
1418358_at 3.6 A 0.828613
1437472_at 41.6 A 0.246094
1423056_at 21.3 A 0.601074
1421061_at 60.8 A 0.27417
1422044_at 150.1 P 0.005859
1435944_s_at 57.1 A 0.533936
1419937_at 111.8 A 0.067627
1419763_at 43.1 A 0.067627
1421036_at 21.9 A 0.533936
1421988_at 69.6 A 0.219482
1415801_at 45.5 A 0.5
1426153_a_at 150.6 P 0.005859
1427610_at 220.4 P 0.001953
1431214_at 531.4 P 0.046143

Total number of rows: 45101

Table truncated, full table size 1211 Kbytes.




Supplementary file Size Download File type/resource
GSM217342_Hfa4wkliver.CEL.gz 3.5 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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