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Sample GSM217344 Query DataSets for GSM217344
Status Public on Dec 01, 2007
Title Liver_HF_week10
Sample type RNA
 
Source name Liver, high-fat
Organism Mus musculus
Characteristics C57/BL6J
Female mice
Biomaterial provider Animal Holding Unit, National University Singapore
Treatment protocol Liver samples comprising identical lobes were quickly removed from each mouse. The liver samples were immediately snap-frozen in liquid nitrogen and stored at -80┬░C.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from the frozen liver tissue of each mouse using Trizol reagent (Life Technologies, 15596-018) and purified using RNeasy Mini Kit (Qiagen, 74104), according to manufacturer's protocol.
Label Biotin
Label protocol Biotinylated cRNA were prepared using the Genechip IVT Labelling Kit (Affymetrix, 900182), according to manufacturer's protocol.
 
Hybridization protocol Following fragmentation, the labeled cRNA were hybridized to the Affymetrix GeneChip® Mouse Genome 430 2.0 chip according to the protocol provided by the manufacturer.
Scan protocol GeneChips were scanned using the Agilent GeneArray Scanner G2500A.
Description Liver tissue from C57BL/6J mice fed on high-fat diets
Data processing The GeneChip Operating Software Version 1.2.0.037 (Affymetrix) was used to analyze the scanned image. The normalization target was set to 500.
 
Submission date Aug 12, 2007
Last update date Dec 29, 2011
Contact name Mei Yen Chan
E-mail(s) m_y_chan3@hotmail.com
Organization name Yong Loo Lin School of Medicine, National University of Singapore
Department Pediatrics
Lab Cardiovascular Genetics
Street address 5 Lower Kent Ridge Road
City Singapore
State/province Singapore
ZIP/Postal code 119074
Country Singapore
 
Platform ID GPL339
Series (1)
GSE8753 Sequential responses to high-fat feeding in an obese mouse model

Data table header descriptions
ID_REF
VALUE Signal
ABS_CALL indicating whether the transcript was present (P), absent (A), or marginal (M)
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 477.386 P 0.00255552
AFFX-BioB-M_at 730.597 P 0.00401721
AFFX-BioB-3_at 277.19 P 0.0151826
AFFX-BioC-5_at 1059.59 P 0.000146581
AFFX-BioC-3_at 599.382 P 0.00010954
AFFX-BioDn-5_at 785.849 P 0.000224668
AFFX-BioDn-3_at 6102.3 P 0.000169227
AFFX-CreX-5_at 10055.8 P 5.16732e-05
AFFX-CreX-3_at 15106.6 P 4.42873e-05
AFFX-DapX-5_at 41.9974 A 0.382599
AFFX-DapX-M_at 64.4336 A 0.327065
AFFX-DapX-3_at 7.92693 A 0.981719
AFFX-LysX-5_at 36.6304 A 0.51489
AFFX-LysX-M_at 65.8668 A 0.631562
AFFX-LysX-3_at 70.7497 A 0.195266
AFFX-PheX-5_at 6.84175 A 0.712257
AFFX-PheX-M_at 5.20298 A 0.868639
AFFX-PheX-3_at 46.6618 A 0.470241
AFFX-ThrX-5_at 26.0913 A 0.659339
AFFX-ThrX-M_at 65.9025 A 0.574038

Total number of rows: 22690

Table truncated, full table size 691 Kbytes.




Supplementary file Size Download File type/resource
GSM217344_High-fat_MOE430A_Chip_20.11.03.CEL.gz 3.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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