|
| Status |
Public on Apr 14, 2008 |
| Title |
embryo_Dnmt3l-/+_8.5dpc_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
whole embryo, Dnmt3l-/+ 8.5dpc maternal methylation-deficient mutant
|
| Organism |
Mus musculus |
| Characteristics |
gender: mixed (six pooled litter mates)
age: 8.5 dpc
tissue: whole embryo
genotype: Dnmt3l-/+ (offspring of a Dnmt3l-/- dam and a wildtype sire) on a C57Bl/6J background
|
| Biomaterial provider |
Deborah Bourc'his
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol (Invitrogen)
|
| Label |
biotin
|
| Label protocol |
Total RNA was quantified on an Agilent Bioanalyzer and 3µg was used to prepare biotin-labeled target cRNA as detailed in the Affymetrix expression manual (http://www.affymetrix.com/Auth/support/downloads/manuals/expression_ever_manual.zip).
|
| |
|
| Hybridization protocol |
The biotinylated target cRNA was purified using a cRNA Cleanup spin column (Affymetrix), fragmented with 5x fragmentation buffer (Affymetrix), and quantified again with the Bioanalyser prior to hybridization. 15 µg of labeled and fragmented target cRNA was used per array. Hybridization, washing and staining were all performed on an Affymetrix Fluidics station 450.
|
| Scan protocol |
An Affymetrix Scanner 3000 was used to generate the raw array image data.
|
| Description |
N/A
|
| Data processing |
The raw array image was transformed into CEL files and analyzed at the probe level using the Affymetrix GCOS (MAS5). The array was normalized to a target (TGT) value of 500.
|
| |
|
| Submission date |
Aug 13, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Reiner Schulz |
| E-mail(s) |
reiner.schulz@kcl.ac.uk
|
| Organization name |
King's College London
|
| Department |
Medical & Molecular Genetics
|
| Lab |
Epigenetics
|
| Street address |
8th floor Guy's Tower
|
| City |
London |
| ZIP/Postal code |
SE1 9RT |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE8756 |
Dnmt3l-/+ (maternal methylation-deficient) vs normal 8.5dpc embryos |
|
| Relations |
| Reanalyzed by |
GSE119085 |
