|
| Status |
Public on Feb 14, 2018 |
| Title |
Long-lived merozoite Plasmodium falciparum rep 4 |
| Sample type |
RNA |
| |
|
| Source name |
long-lived merozoite Plasmodium falciparum, merozoite stage
|
| Organism |
Plasmodium falciparum |
| Characteristics |
strain/background: LLM
phenotype: long-lived merozoite
developmental stage: merozoite
|
| Treatment protocol |
WT vs. LLM.
|
| Growth protocol |
Merozoites were passed through a 5% sucrose cushion at 1500 x g for 7 mins, followed by a PBS wash at 5000 x g for 10 mins.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the RNeasy 96 kit according to manufacturer's recommendations.
|
| Label |
biotin
|
| Label protocol |
Labeling was performed using the Ovation Pico WTA system (Nugen Inc., San Carlos, CA).
|
| |
|
| Hybridization protocol |
Samples were hybridized onto Affymetrix GeneChip Plasmodium/Anopheles arrays according to manufacturer's recommendations.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 7GPlus.
|
| Description |
Gene expression data from long-lived merozoite Plasmodium falciparum, merozoite stage rep 4.
|
| Data processing |
The data were analyzed with Expression Console (EC) 1.4 using Affymetrix default analysis with a Scale factor normalization method (mas5).
|
| |
|
| Submission date |
May 24, 2016 |
| Last update date |
May 20, 2018 |
| Contact name |
Dan Sturdevant |
| E-mail(s) |
dsturdevant@niaid.nih.gov
|
| Phone |
4063639248
|
| Organization name |
NIH
|
| Department |
NIAID
|
| Lab |
RTS
|
| Street address |
903 S 4th street
|
| City |
Hamilton |
| State/province |
MT |
| ZIP/Postal code |
59840 |
| Country |
USA |
| |
|
| Platform ID |
GPL1321 |
| Series (1) |
| GSE81818 |
Profiling γ-irradiated invasive Plasmodium falciparum merozoites using a systems biology approach |
|
