|
Status |
Public on Feb 16, 2017 |
Title |
IGG |
Sample type |
SRA |
|
|
Source name |
breast tumor
|
Organism |
Homo sapiens |
Characteristics |
cell line: MCF-7 from NCI-60 antibody: IgG control
|
Treatment protocol |
CD95 stimulation was done with IgG and APO-1 100ngr/ml with protein-A 1ng/ml.
|
Growth protocol |
Cell lines were maintained in RPMI supplemented with 10% FBS.
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with anti-STAT1 antibody
|
|
|
Library strategy |
ChIP-Seq |
Library source |
genomic |
Library selection |
ChIP |
Instrument model |
Illumina HiScanSQ |
|
|
Description |
Chromatin IP
|
Data processing |
Reads were aligned to the hg19 reference human genome using bwa v0.6.1 Peak-calling by HOMER v4.1 Genome_build: hg19 Genome_build: hg19 Supplementary_files_format_and_content: bedGraph file and peak txt file
|
|
|
Submission date |
May 25, 2016 |
Last update date |
Sep 08, 2022 |
Contact name |
Marcus Peter |
E-mail(s) |
m-peter@northwestern.edu
|
Organization name |
Northwestern University Feinberg School of Medicine
|
Street address |
303 East Superior Street, Lurie 6-123
|
City |
Chicago |
State/province |
IL |
ZIP/Postal code |
60611 |
Country |
USA |
|
|
Platform ID |
GPL15456 |
Series (2) |
GSE81859 |
CD95/Fas increases stemness in cancer cells by inducing a STAT1 dependent Type I interferon response [ChIP-seq] |
GSE81860 |
CD95/Fas increases stemness in cancer cells by inducing a STAT1 dependent Type I interferon response |
|
Relations |
BioSample |
SAMN05173134 |
SRA |
SRX1799016 |