|
Status |
Public on Sep 15, 2007 |
Title |
hAT1R tg Replicate1 |
Sample type |
RNA |
|
|
Source name |
heart tissue from C57BL/6 mouse over expressiong hAT1R
|
Organism |
Mus musculus |
Characteristics |
hAT1R transgenic mouse heart
|
Extracted molecule |
total RNA |
Extraction protocol |
RNEasy (Qiagen) Kit was used for total RNA extraction from heart tissue. For homogenization,
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
|
|
Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
Scan protocol |
GeneChips were scanned using the GeneChip® Scanner 3000.
|
Description |
Gene expression data from C57BL/6 mouse over expressiong hAT1R
|
Data processing |
The data were preprocessed with GCRMA package provided in Bioconductor and quantile normalization as normalization method.
|
|
|
Submission date |
Aug 14, 2007 |
Last update date |
Aug 28, 2018 |
Contact name |
Venkata Suresh Kumar surampudi |
E-mail(s) |
kumars@ccf.org
|
Phone |
216-445-6636
|
Organization name |
Lerner Research Institute
|
Department |
Molecular Cardiology
|
Lab |
Karnik Lab
|
Street address |
9500 Euclid Avenue
|
City |
Cleveland |
State/province |
OH |
ZIP/Postal code |
44195 |
Country |
USA |
|
|
Platform ID |
GPL1261 |
Series (1) |
GSE8771 |
Expression data from hAT1R transgenic and non-transgenic C57BL/6 mouse heart tissue |
|
Relations |
Reanalyzed by |
GSE119085 |