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Sample GSM217869 Query DataSets for GSM217869
Status Public on Sep 15, 2007
Title hAT1R tg Replicate2
Sample type RNA
 
Source name heart tissue from C57BL/6 mouse over expressiong hAT1R
Organism Mus musculus
Characteristics hAT1R transgenic mouse heart
Extracted molecule total RNA
Extraction protocol RNEasy (Qiagen) Kit was used for total RNA extraction from heart tissue. For homogenization,
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the GeneChip® Scanner 3000.
Description Gene expression data from C57BL/6 mouse over expressiong hAT1R
Data processing The data were preprocessed with GCRMA package provided in Bioconductor and quantile normalization as normalization method.
 
Submission date Aug 14, 2007
Last update date Aug 28, 2018
Contact name Venkata Suresh Kumar surampudi
E-mail(s) kumars@ccf.org
Phone 216-445-6636
Organization name Lerner Research Institute
Department Molecular Cardiology
Lab Karnik Lab
Street address 9500 Euclid Avenue
City Cleveland
State/province OH
ZIP/Postal code 44195
Country USA
 
Platform ID GPL1261
Series (1)
GSE8771 Expression data from hAT1R transgenic and non-transgenic C57BL/6 mouse heart tissue
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE GCRMA signal intensity

Data table
ID_REF VALUE
1415670_at 9.488938534
1415671_at 9.498997584
1415672_at 10.28475665
1415673_at 6.589302732
1415674_a_at 8.79486786
1415675_at 6.751362302
1415676_a_at 11.32715623
1415677_at 9.103922002
1415678_at 10.78335263
1415679_at 10.35993428
1415680_at 8.396573876
1415681_at 9.646695544
1415682_at 8.083126703
1415683_at 10.47524112
1415684_at 7.976123896
1415685_at 9.160032738
1415686_at 8.989486006
1415687_a_at 12.60742944
1415688_at 10.69844551
1415689_s_at 7.064520715

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.




Supplementary file Size Download File type/resource
GSM217869.CEL.gz 3.7 Mb (ftp)(http) CEL
GSM217869.EXP.gz 398 b (ftp)(http) EXP
Processed data included within Sample table
Raw data provided as supplementary file

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