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Sample GSM217870 Query DataSets for GSM217870
Status Public on Sep 15, 2007
Title hAT1R tg Replicate3
Sample type RNA
 
Source name heart tissue from C57BL/6 mouse over expressiong hAT1R
Organism Mus musculus
Characteristics hAT1R transgenic mouse heart
Extracted molecule total RNA
Extraction protocol RNEasy (Qiagen) Kit was used for total RNA extraction from heart tissue. For homogenization,
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the GeneChip® Scanner 3000.
Description Gene expression data from C57BL/6 mouse over expressiong hAT1R
Data processing The data were preprocessed with GCRMA package provided in Bioconductor and quantile normalization as normalization method.
 
Submission date Aug 14, 2007
Last update date Aug 28, 2018
Contact name Venkata Suresh Kumar surampudi
E-mail(s) kumars@ccf.org
Phone 216-445-6636
Organization name Lerner Research Institute
Department Molecular Cardiology
Lab Karnik Lab
Street address 9500 Euclid Avenue
City Cleveland
State/province OH
ZIP/Postal code 44195
Country USA
 
Platform ID GPL1261
Series (1)
GSE8771 Expression data from hAT1R transgenic and non-transgenic C57BL/6 mouse heart tissue
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE GCRMA signal intensity

Data table
ID_REF VALUE
1415670_at 9.662239489
1415671_at 9.737762698
1415672_at 10.29251798
1415673_at 6.542151487
1415674_a_at 8.924083245
1415675_at 7.00399248
1415676_a_at 11.38309038
1415677_at 9.153800936
1415678_at 10.94442701
1415679_at 10.43739912
1415680_at 8.559801429
1415681_at 9.687100113
1415682_at 8.214128398
1415683_at 10.66764297
1415684_at 7.967887598
1415685_at 9.123712607
1415686_at 9.172128728
1415687_a_at 12.66361478
1415688_at 10.74652225
1415689_s_at 7.198041387

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.




Supplementary file Size Download File type/resource
GSM217870.CEL.gz 3.7 Mb (ftp)(http) CEL
GSM217870.EXP.gz 396 b (ftp)(http) EXP
Processed data included within Sample table
Raw data provided as supplementary file

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