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Sample GSM217871 Query DataSets for GSM217871
Status Public on Sep 15, 2007
Title Non transgenic Replicate1
Sample type RNA
 
Source name heart tissue from normal C57BL/6 mouse
Organism Mus musculus
Characteristics non-transgenic mouse heart
Extracted molecule total RNA
Extraction protocol RNEasy (Qiagen) Kit was used for total RNA extraction from heart tissue. For homogenization,
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the GeneChip® Scanner 3000.
Description Gene expression data from normal C57BL/6 mouse
Data processing The data were preprocessed with GCRMA package provided in Bioconductor and quantile normalization as normalization method.
 
Submission date Aug 14, 2007
Last update date Aug 28, 2018
Contact name Venkata Suresh Kumar surampudi
E-mail(s) kumars@ccf.org
Phone 216-445-6636
Organization name Lerner Research Institute
Department Molecular Cardiology
Lab Karnik Lab
Street address 9500 Euclid Avenue
City Cleveland
State/province OH
ZIP/Postal code 44195
Country USA
 
Platform ID GPL1261
Series (1)
GSE8771 Expression data from hAT1R transgenic and non-transgenic C57BL/6 mouse heart tissue
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE GCRMA signal intensity

Data table
ID_REF VALUE
1415670_at 9.335167785
1415671_at 9.67801044
1415672_at 10.28067867
1415673_at 6.805063753
1415674_a_at 8.69089326
1415675_at 6.746257275
1415676_a_at 11.42654044
1415677_at 9.463002623
1415678_at 10.77065664
1415679_at 10.36242228
1415680_at 8.482767924
1415681_at 9.956995213
1415682_at 8.204184201
1415683_at 10.31357079
1415684_at 7.950777789
1415685_at 9.436369803
1415686_at 9.22007263
1415687_a_at 12.79994561
1415688_at 10.56002536
1415689_s_at 7.331196516

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.




Supplementary file Size Download File type/resource
GSM217871.CEL.gz 3.5 Mb (ftp)(http) CEL
GSM217871.EXP.gz 400 b (ftp)(http) EXP
Processed data included within Sample table
Raw data provided as supplementary file

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