|
| Status |
Public on Sep 15, 2007 |
| Title |
Non transgenic Replicate3 |
| Sample type |
RNA |
| |
|
| Source name |
heart tissue from normal C57BL/6 mouse
|
| Organism |
Mus musculus |
| Characteristics |
non-transgenic mouse heart
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNEasy (Qiagen) Kit was used for total RNA extraction from heart tissue. For homogenization,
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the GeneChip® Scanner 3000.
|
| Description |
Gene expression data from normal C57BL/6 mouse
|
| Data processing |
The data were preprocessed with GCRMA package provided in Bioconductor and quantile normalization as normalization method.
|
| |
|
| Submission date |
Aug 14, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Venkata Suresh Kumar surampudi |
| E-mail(s) |
kumars@ccf.org
|
| Phone |
216-445-6636
|
| Organization name |
Lerner Research Institute
|
| Department |
Molecular Cardiology
|
| Lab |
Karnik Lab
|
| Street address |
9500 Euclid Avenue
|
| City |
Cleveland |
| State/province |
OH |
| ZIP/Postal code |
44195 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE8771 |
Expression data from hAT1R transgenic and non-transgenic C57BL/6 mouse heart tissue |
|
| Relations |
| Reanalyzed by |
GSE119085 |
