NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM2178992 Query DataSets for GSM2178992
Status Public on May 28, 2016
Title Ep
Sample type RNA
 
Source name epidydimal adipose tissue
Organism Mus musculus
Characteristics strain: C57BL/6
tissue: epidydimal adipose tissue
age: post natal month 2-3
Extracted molecule total RNA
Extraction protocol Brown adipose tissue, inguinal adipose tissue, epidydimal adipose tissue, gastrocnemius muscle, and soleus muscle were removed, flash frozen on dry ice, and RNA was harvested using Trizol reagent. RNasey Mini Kit(Qiagen p/n 74104) was used to obtain 1 ug of total RNA for miRNA labeling.
Label Cy3
Label protocol miRCURYTM Array Power Labeling kit (Cat #208032-A, Exiqon) was used for miRNA labeling.
 
Hybridization protocol miRNA array hybridization was performed using miRCURYTM Array Hybridization buffer and Wash buffer
Scan protocol Axon GenePix 4000B microarray scanner and GenePix pro V6.0 were used for miRNA array scanning and analysis.
Data processing GenePix pro V6.0 is used to read the raw intensity of the image. The intensity of green signal is calculated after background subtraction and four Replicated spots of each probe on the same slide have been calculated the median. We use Median Normalization Method to obtain “Normalized Data”, Normalized Data = (Foreground-Background) ­/ ­median, the median is 50 percent quantile of microRNA intensity which is larger than 50 in all samples after background correction.
 
Submission date May 27, 2016
Last update date May 28, 2016
Contact name Hao Ying
E-mail(s) yinghao@sibs.ac.cn
Phone 862154920247
Organization name SIBS,CAS
Department INS
Street address 320 YUEYANG ROAD
City Shanghai
ZIP/Postal code 200031
Country China
 
Platform ID GPL7723
Series (1)
GSE81976 microRNA profiling of different adipose tissues and muscles

Data table header descriptions
ID_REF
VALUE Normalized signal intensity. We use Median Normalization Method to do array normalizaion.

Data table
ID_REF VALUE
4610 8.098484848
11013 0.025252525
11102 0.01010101
19604 0.003787879
17306 0.013888889
17529 0.003787879
17660 0.006313131
17940 0.002525253
5740 15.70328283
11020 4.965909091
11111 0.001262626
11222 0.002525253
17327 0.046717172
17536
17732 0.170454545
17953 0.064393939
10306 1.130050505
11024 3.386363636
11123 0.007575758
11277 0.005050505

Total number of rows: 1973

Table truncated, full table size 31 Kbytes.




Supplementary data files not provided
Processed data included within Sample table
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap